Inhibitory effects of pomelo on the metabolism of tacrolimus and the activities of CYP3A4 and P-glycoprotein

Kanoko Egashira, Hisakazu Ohtani, Suwako Itoh, Noriko Koyabu, Masayuki Tsujimoto, Hideyasu Murakami, Yasufumi Sawada

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We recently reported a case of increase in the blood level of tacrolimus following intake of pomelo in a renal transplant recipient. To clarify the mechanism of this increase in the blood level of tacrolimus, we investigated the effect of pomelo juice extract on the activities of CYP3A4 and P-glycoprotein, in comparison with that of extract of grapefruit juice (GFJ). The 10% ethyl acetate extracts of the juice of three pomelos of different origins (Banpeiyu, pomelo I; Hirado Buntan, pomelo II; and Tosa Buntan, pomelo III) and GFJ significantly inhibited 6β-hydroxylation of testosterone in human liver microsomes by 76.4, 67.2, 37.5, and 83.9%, respectively. The extract of pomelo I was as potent as that of GFJ. The metabolism of tacrolimus itself was also inhibited by the extract of pomelo I, as well as that of GFJ. Furthermore, the inhibition of both 6β-hydroxylation of testosterone and metabolism of tacrolimus by pomelo I and GFJ was preincubation time-dependent. On the other hand, the extract of pomelo I had little effect on the transcellular transport of tacrolimus or [3H]digoxin across a monolayer of LLC-GA5-COL150 cells (a porcine kidney epithelial cell line, LLC-PK1, transfected with human MDR1 cDNA and over-expressing human P-glycoprotein). In conclusion, pomelo constituents inhibit the activity of CYP3A4 and may thereby produce an increase in the blood level of tacrolimus.

Original languageEnglish
Pages (from-to)828-833
Number of pages6
JournalDrug Metabolism and Disposition
Volume32
Issue number8
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

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Cytochrome P-450 CYP3A
Citrus paradisi
P-Glycoprotein
Tacrolimus
Metabolism
Hydroxylation
Blood
Testosterone
Enzyme inhibition
Kidney
Transcytosis
Transplants
Digoxin
Liver Microsomes
Liver
Monolayers
Swine
Complementary DNA
Epithelial Cells
Cell Line

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

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Inhibitory effects of pomelo on the metabolism of tacrolimus and the activities of CYP3A4 and P-glycoprotein. / Egashira, Kanoko; Ohtani, Hisakazu; Itoh, Suwako; Koyabu, Noriko; Tsujimoto, Masayuki; Murakami, Hideyasu; Sawada, Yasufumi.

In: Drug Metabolism and Disposition, Vol. 32, No. 8, 08.2004, p. 828-833.

Research output: Contribution to journalArticle

Egashira, Kanoko ; Ohtani, Hisakazu ; Itoh, Suwako ; Koyabu, Noriko ; Tsujimoto, Masayuki ; Murakami, Hideyasu ; Sawada, Yasufumi. / Inhibitory effects of pomelo on the metabolism of tacrolimus and the activities of CYP3A4 and P-glycoprotein. In: Drug Metabolism and Disposition. 2004 ; Vol. 32, No. 8. pp. 828-833.
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