Inhibitory effects of various beverages on the sulfoconjugation of 17β-estradiol in human colon carcinoma caco-2 cells

Ayako Saruwatari, Marina Isshiki, Hiroomi Tamura

Research output: Contribution to journalArticle

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Abstract

To investigate the possible effects of different beverages in the gastrointestinal tract on the sulfation of estrogen, which is a major hormone and prototype substrate for the human sulfotransferases (SULT), we analyzed the effects of these substances upon the sulfate conjugation of 17β-estradiol (E2) in the human colon carcinoma cell line Caco-2. Sulfoconjugation activity toward E2 was measured by incubating 20 nM E2 with Caco-2 cells in the presence (5% (v/v)) of each beverage. Among the 35 beverages analyzed, four (aronia, blueberry, coffee, and peppermint) exhibited strong inhibitory effects on E2 sulfation within Caco-2 cells (IC50 values ranging from 1.9 to 4.4% (v/v)). These active beverages also strongly inhibited the cytosolic estrogen SULT activity of Caco-2 cells in vitro (IC50 values ranging from 0.18 to 0.3% (v/v)). These inhibitory activities were extractable with ethyl acetate but not hexane or n-butanol, indicating that the molecules responsible are moderately lipophilic. Coffee was found to be the most potent inhibitor but the major constituents of this beverage, caffeic acid, caffeine, and chlorogenic acid, did not show any effects on estrogen SULT activity. Kinetic analyses further indicated that the mode of inhibition by coffee is competitive. A possible relationship between the inhibition of estrogen SULT activity by coffee in the gastrointestinal tract and the reported reduction of colon cancer incidence in women who consume coffee is discussed.

Original languageEnglish
Pages (from-to)2131-2136
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume31
Issue number11
DOIs
Publication statusPublished - 2008 Nov

Fingerprint

Caco-2 Cells
Coffee
Beverages
Estradiol
Colon
Carcinoma
Inhibitory Concentration 50
Gastrointestinal Tract
Photinia
Mentha piperita
Blueberry Plants
Sulfotransferases
Chlorogenic Acid
1-Butanol
Hexanes
Caffeine
Colonic Neoplasms
Estrogens
Hormones
Cell Line

Keywords

  • Caco-2
  • Coffee
  • Estrogen
  • Sulfotransferase

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Inhibitory effects of various beverages on the sulfoconjugation of 17β-estradiol in human colon carcinoma caco-2 cells. / Saruwatari, Ayako; Isshiki, Marina; Tamura, Hiroomi.

In: Biological and Pharmaceutical Bulletin, Vol. 31, No. 11, 11.2008, p. 2131-2136.

Research output: Contribution to journalArticle

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