Inhibitory mechanisms of glycoprotein fraction derived from Miscanthus sinensis for the immediate phase response of an IgE-mediated cutaneous reaction

Chie Watanabe, Taku Satoh, Eiichi Tahara, Koji Murakami, Kazuko Hayashi, Kouji Hase, Tsugunobu Andoh, Yasushi Kuraishi, Shigetoshi Kadota, Hiroichi Nagai, Ikuo Saiki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We investigated the inhibitory effect of the glycoprotein fraction (fraction 2) extracted from Miscanthus sinensis ANDERSSON (M. sinensis) on biphasic cutaneous reactions in mice passively sensitized with IgE. Biphasic skin reactions with peak responses at 1 (IPR, immediate phase reaction) and 24h (LPR, late phase reaction) were caused by passive sensitization with an anti-dinitrophenol IgE monoclonal antibody (anti-DNP IgE mAb) followed by an epicutaneous challenge of 0.1% dinitrofluorobenzene (DNFB) in 100% ethanol. Intraperitoneal injection of fraction 2 before the DNFB challenge significantly inhibited the biphasic ear swelling response in passively sensitized mice in a dose-dependent manner (1-30 mg/kg). We also found that fraction 2 was effective at inhibiting the vascular permeability in mouse ear induced by an injection of compound 48/80, histamine or serotonin. In addition, fraction 2 inhibited scratching behavior as well as ear edema observed within 2 h after DNFB challenge. Marked inhibition was observed in both passively sensitized and non-sensitized mice. The locomotor activity of mice was also reduced by the administration of fraction 2 as well as by diphenhydramine. These results suggest that the inhibitory effect of glycoprotein fraction 2 of M. sinensis on an IgE-mediated allergic inflammatory reaction is due to the protection of mediator-induced vascular permeability and that in addition to the inhibition of an inflammatory reaction; a sedative action is responsible for the inhibition of allergy- induced scratching responses.

Original languageEnglish
Pages (from-to)26-30
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number1
Publication statusPublished - 1999 Jan
Externally publishedYes

Fingerprint

Immunoglobulin E
Dinitrofluorobenzene
Glycoproteins
Skin
Ear
Capillary Permeability
Hypersensitivity
Dinitrophenols
p-Methoxy-N-methylphenethylamine
Diphenhydramine
Locomotion
Intraperitoneal Injections
Hypnotics and Sedatives
Histamine
Edema
Serotonin
Ethanol
Monoclonal Antibodies
Injections
Inhibition (Psychology)

Keywords

  • IgE-mediated skin reaction
  • Miscanthus sinensis
  • Scratching
  • Sedation
  • Vascular permeability

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Inhibitory mechanisms of glycoprotein fraction derived from Miscanthus sinensis for the immediate phase response of an IgE-mediated cutaneous reaction. / Watanabe, Chie; Satoh, Taku; Tahara, Eiichi; Murakami, Koji; Hayashi, Kazuko; Hase, Kouji; Andoh, Tsugunobu; Kuraishi, Yasushi; Kadota, Shigetoshi; Nagai, Hiroichi; Saiki, Ikuo.

In: Biological and Pharmaceutical Bulletin, Vol. 22, No. 1, 01.1999, p. 26-30.

Research output: Contribution to journalArticle

Watanabe, C, Satoh, T, Tahara, E, Murakami, K, Hayashi, K, Hase, K, Andoh, T, Kuraishi, Y, Kadota, S, Nagai, H & Saiki, I 1999, 'Inhibitory mechanisms of glycoprotein fraction derived from Miscanthus sinensis for the immediate phase response of an IgE-mediated cutaneous reaction', Biological and Pharmaceutical Bulletin, vol. 22, no. 1, pp. 26-30.
Watanabe, Chie ; Satoh, Taku ; Tahara, Eiichi ; Murakami, Koji ; Hayashi, Kazuko ; Hase, Kouji ; Andoh, Tsugunobu ; Kuraishi, Yasushi ; Kadota, Shigetoshi ; Nagai, Hiroichi ; Saiki, Ikuo. / Inhibitory mechanisms of glycoprotein fraction derived from Miscanthus sinensis for the immediate phase response of an IgE-mediated cutaneous reaction. In: Biological and Pharmaceutical Bulletin. 1999 ; Vol. 22, No. 1. pp. 26-30.
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