Inhibitory regulation of serum factor(s)-caused ornithine decarboxylase induction by the protein kinase C system in A431 human epidermoid carcinoma cells

T. Nakadate, S. Yamamoto, Y. Kamegaya, M. Ishihara, Eriko Yokota, H. Jiang, R. Kato

Research output: Contribution to journalArticle

Abstract

Replacement of the culture medium with fresh medium containing 10% fetal calf serum caused ornithine decarboxylase (ODC) induction in A431 human epidermoid carcinoma cells. Two peaks of ODC activity were observed at 5 and 14 hr after the medium replacement. The peak activity observed at 5 hr was more prominent than that at 14 hr. The first peak of ODC induction was suppressed by a potent protein kinase C activator, 12-O-tetradecanoylphorbol-13-acetate (TPA), in a concentration-dependent manner. The second peak, however, was not suppressed by TPA. Other potent protein kinase C activators, such as mezerein and 12-O-retinoylphorbol-13-acetate, also suppressed the first peak of ODC induction. Synthetic diacylglycerols, 1,2-dioctanoyl-sn-glycerol and 1-oleoyl-2-acetylglycerol, did not inhibit the serum factor(s)-caused ODC induction. Phorbol-13-acetate, an inactive phorbol ester, also failed to inhibit the ODC induction. The growth of A431 cells was slightly suppressed by TPA. In protein kinase C down-regulated cells, TPA failed to inhibit the serum factor(s)-caused ODC induction. These results suggest that the serum factor(s)-caused ODC induction in A431 cells is negatively regulated by the protein kinase C system, which may not be activated by exogenous diacylglycerols.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalJapanese Journal of Pharmacology
Volume56
Issue number2
Publication statusPublished - 1991

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Ornithine Decarboxylase
Protein Kinase C
Squamous Cell Carcinoma
Tetradecanoylphorbol Acetate
Serum
Diglycerides
Phorbol Esters
Culture Media
Acetates
Growth

ASJC Scopus subject areas

  • Pharmacology

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Inhibitory regulation of serum factor(s)-caused ornithine decarboxylase induction by the protein kinase C system in A431 human epidermoid carcinoma cells. / Nakadate, T.; Yamamoto, S.; Kamegaya, Y.; Ishihara, M.; Yokota, Eriko; Jiang, H.; Kato, R.

In: Japanese Journal of Pharmacology, Vol. 56, No. 2, 1991, p. 203-211.

Research output: Contribution to journalArticle

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AU - Kato, R.

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