Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade

Yukiko Gotoh, Norihisa Masuyama, Karen Dell, Kyoko Shirakabe, Eisuke Nishida

Research output: Contribution to journalArticle

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Abstract

Mitogen-activated protein kinase (MAPK) and MAPK kinase (MAPKK) are activated during Xenopus oocyte maturation concomitant with the activation of maturation promoting factor (MPF). We reported previously that an anti-MAPKK neutralizing antibody inhibited progesterone- or Mos- induced initiation of oocyte maturation. Here, we show that injection of CL100 (also called MAPK phosphatase-1) into immature oocytes inhibited progesterone-induced oocyte maturation as well as MAPK activation and that injection of mRNA encoding a constitutively active MAPKK induced activation of histone H1 kinase and germinal vesicle breakdown in the absence of progesterone. Injection of recombinant STE11 protein (a yeast MAPKK kinase) also induced initiation of oocyte maturation. These data support the idea that the MAPKK/MAPK cascade plays an important role in oocyte maturation. Interestingly, injection of the active MAPKK mRNA or the STE11 protein resulted in induction and accumulation of Mos protein. Furthermore, in the presence of cycloheximide, the STE11-induced activation of MPF as well as the induction and accumulation of Mos was blocked, and the activation of MAPK was greatly reduced. The increase in Mos protein and the activation of MAPK by injecting cyclin A protein into immature oocytes were both blocked also by cycloheximide treatment. These results are consistent with an idea that there may exist a positive feedback loop consisting of Mos, the MAPKK/MAPK cascade, and MPF, which may be important for the initiation of oocyte maturation induced by progesterone.

Original languageEnglish
Pages (from-to)25898-25904
Number of pages7
JournalJournal of Biological Chemistry
Volume270
Issue number43
Publication statusPublished - 1995 Oct 27

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Mitogen-Activated Protein Kinase Kinases
Xenopus
Mitogen-Activated Protein Kinases
Oocytes
Chemical activation
Maturation-Promoting Factor
Progesterone
Cycloheximide
Injections
Proteins
Mitogen-Activated Protein Kinase Phosphatases
MAP Kinase Kinase Kinases
Cyclin A
Recombinant proteins
Messenger RNA
Dual Specificity Phosphatase 1
Neutralizing Antibodies
Yeast
Phosphotransferases
Recombinant Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gotoh, Y., Masuyama, N., Dell, K., Shirakabe, K., & Nishida, E. (1995). Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade. Journal of Biological Chemistry, 270(43), 25898-25904.

Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade. / Gotoh, Yukiko; Masuyama, Norihisa; Dell, Karen; Shirakabe, Kyoko; Nishida, Eisuke.

In: Journal of Biological Chemistry, Vol. 270, No. 43, 27.10.1995, p. 25898-25904.

Research output: Contribution to journalArticle

Gotoh, Y, Masuyama, N, Dell, K, Shirakabe, K & Nishida, E 1995, 'Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade', Journal of Biological Chemistry, vol. 270, no. 43, pp. 25898-25904.
Gotoh Y, Masuyama N, Dell K, Shirakabe K, Nishida E. Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade. Journal of Biological Chemistry. 1995 Oct 27;270(43):25898-25904.
Gotoh, Yukiko ; Masuyama, Norihisa ; Dell, Karen ; Shirakabe, Kyoko ; Nishida, Eisuke. / Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 43. pp. 25898-25904.
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