Injection of isolated centriolar complex to induce mitosis in starfish eggs

Hisafumi Yamada, Ritsu Kuraishi, Reiko M. Amikura, Hiroshi Nagano

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

When centriolar complex isolated from starfish spermatozoa was injected into starfish immature oocytes (fully grown, germinal vesicle stage) followed by treating the latter with 1‐methyladenine, mitotic events such as condensation and division of chromosomes of the female pronucleus and cytokinesis following completion of meiosis were observed. No cortical reaction detected in the oocytes. Essentially the same was noted for mature oocytes (pronuclear stage) into which the centriolar complex had been injected. The oocytes that had received sperm tail fraction or buffer alone did not initiate cleavage. It would thus appear that sperm centriolar complex is significantly essential to the initiation of cleavage.

Original languageEnglish
Pages (from-to)356-360
Number of pages5
JournalMolecular reproduction and development
Volume26
Issue number4
DOIs
Publication statusPublished - 1990 Aug
Externally publishedYes

Fingerprint

Starfish
Mitosis
Eggs
Oocytes
Injections
Spermatozoa
Sperm Tail
Cytokinesis
Meiosis
Buffers
Chromosomes

Keywords

  • Initiation of mitosis
  • Oocytes
  • Sperm centriolar complex

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Injection of isolated centriolar complex to induce mitosis in starfish eggs. / Yamada, Hisafumi; Kuraishi, Ritsu; Amikura, Reiko M.; Nagano, Hiroshi.

In: Molecular reproduction and development, Vol. 26, No. 4, 08.1990, p. 356-360.

Research output: Contribution to journalArticle

Yamada, Hisafumi ; Kuraishi, Ritsu ; Amikura, Reiko M. ; Nagano, Hiroshi. / Injection of isolated centriolar complex to induce mitosis in starfish eggs. In: Molecular reproduction and development. 1990 ; Vol. 26, No. 4. pp. 356-360.
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