Innervation of the tibial epiphysis through the intercondylar foramen

Koichi Matsuo, Shuting Ji, Ayako Miya, Masaki Yoda, Yuzuru Hamada, Tomoya Tanaka, Ryoko Takao-Kawabata, Katsuhiro Kawaai, Yukiko Kuroda, Shinsuke Shibata

Research output: Contribution to journalArticle

Abstract

The periosteum and mineralized bone are innervated by nerves that sense pain. These include both myelinated and unmyelinated neurons with either free nerve endings or bearing nociceptors. Parasympathetic and sympathetic autonomic nerves also innervate bone. However, little is known about the route sensory nerves take leaving the epiphyses of long bones at the adult knee joint. Here, we used transgenic mice that express fluorescent Venus protein in Schwann cells (Sox10-Venus mice) to visualize myelinated and unmyelinated nerves in the tibial epiphysis. Immunofluorescence to detect a pan-neuronal marker and the sensory neuron markers calcitonin gene-related peptide (CGRP) and tropomyosin receptor kinase A (TrkA) also revealed Schwann cell-associated sensory neurons. Foramina in the intercondylar area of the tibia were conserved between rodents and primates. Venus-labeled fibers were detected within bone marrow of the proximal epiphysis, exited through foramina along with blood vessels in the intercondylar area of the tibia, and joined Venus-labeled fibers of the synovial membrane and meniscus. These data suggest that innervation of the subchondral plate and trabecular bone within the tibial epiphysis carries pain signals from the knee joint to the brain through intercondylar foramina.

LanguageEnglish
Pages297-304
Number of pages8
JournalBone
Volume120
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

Venus
Epiphyses
Schwann Cells
Sensory Receptor Cells
Knee Joint
Tibia
Bone and Bones
Calcitonin Gene-Related Peptide Receptors
Autonomic Pathways
Tibial Nerve
Periosteum
Nociceptors
Nerve Endings
Synovial Membrane
Neuralgia
Primates
Transgenic Mice
Fluorescent Antibody Technique
Blood Vessels
Rodentia

Keywords

  • Epiphysis
  • Foramen
  • Knee joint
  • Schwann cells
  • Sensory neuron
  • Tibia

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Innervation of the tibial epiphysis through the intercondylar foramen. / Matsuo, Koichi; Ji, Shuting; Miya, Ayako; Yoda, Masaki; Hamada, Yuzuru; Tanaka, Tomoya; Takao-Kawabata, Ryoko; Kawaai, Katsuhiro; Kuroda, Yukiko; Shibata, Shinsuke.

In: Bone, Vol. 120, 01.03.2019, p. 297-304.

Research output: Contribution to journalArticle

Matsuo, K, Ji, S, Miya, A, Yoda, M, Hamada, Y, Tanaka, T, Takao-Kawabata, R, Kawaai, K, Kuroda, Y & Shibata, S 2019, 'Innervation of the tibial epiphysis through the intercondylar foramen' Bone, vol. 120, pp. 297-304. https://doi.org/10.1016/j.bone.2018.11.007
Matsuo, Koichi ; Ji, Shuting ; Miya, Ayako ; Yoda, Masaki ; Hamada, Yuzuru ; Tanaka, Tomoya ; Takao-Kawabata, Ryoko ; Kawaai, Katsuhiro ; Kuroda, Yukiko ; Shibata, Shinsuke. / Innervation of the tibial epiphysis through the intercondylar foramen. In: Bone. 2019 ; Vol. 120. pp. 297-304.
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