Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome: An efficient selection method

Rie Uotsu-Tomita, Shinya Kaneko, Kenji Tsuge, Mitsuhiro Itaya

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.

Original languageEnglish
Pages (from-to)1036-1039
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number5
DOIs
Publication statusPublished - 2005 May
Externally publishedYes

Fingerprint

DNA sequences
Bacilli
selection methods
Bacillus subtilis
Genes
Genome
mutation
nucleotide sequences
Mutation
loci
Neomycin
genome
neomycin
genomics
methodology

Keywords

  • Bacillus subtilis genome
  • Inositol
  • pBR322
  • Phleomycin
  • Transformation

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome : An efficient selection method. / Uotsu-Tomita, Rie; Kaneko, Shinya; Tsuge, Kenji; Itaya, Mitsuhiro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 5, 05.2005, p. 1036-1039.

Research output: Contribution to journalArticle

Uotsu-Tomita, Rie ; Kaneko, Shinya ; Tsuge, Kenji ; Itaya, Mitsuhiro. / Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome : An efficient selection method. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 5. pp. 1036-1039.
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