Insertional gene fusion technology

Nobuhide Doi, Hiroshi Yanagawa

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The classical 'end to end' gene fusion technique has widely been used for monitoring gene expression, biological screening and purification of recombinant proteins. Recent progress with the 'insertional' gene fusion approach, on the other hand, has demonstrated that this technique can be utilized for membrane protein topology analysis, display of randomized protein libraries and design of biosensor proteins. In this review, we describe examples of insertional gene fusion and compare the old and new gene fusion techniques. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalFEBS Letters
Volume457
Issue number1
DOIs
Publication statusPublished - 1999 Aug 20
Externally publishedYes

Keywords

  • Biosensor
  • Directed evolution
  • Domain insertion
  • Green fluorescent protein
  • Membrane protein topology
  • Protein engineering

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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