Insulin-like growth factor binding protein-4 accumulation is negatively correlated with growth rate in TM-3 cells

Tomonobu Hasegawa, Y. Hasegawa, R. G. Rosenfeld, P. Cohen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cellular growth is controlled by multiple regulators, including the insulin-like growth factors (IGFs). In some cells, the IGF binding proteins (IGFBPs) are thought to be inhibitory molecules for cell growth and may be related to the process of contact inhibition. In the TM-3 (mouse Leydig) cell line, IGFBP-4 is the major IGFBP secreted into conditioned media (CM), as we have reported. In this study, we investigated cell growth, the peptide levels of IGFBP-4 in CM, and the inverse relationship between IGFBP-4 accumulation and cell growth rate. Quantification of TM-3 growth in serum-containing media demonstrated that TM-3 cell number gradually rose after plating, and plateaued when cells became confluent. The rate of cell growth fell gradually, and net cell growth stopped when cells reached confluency. IGFBP- 4 peptide levels in CM, as measured by Western ligand blot, rose gradually during the culture period and plateaued when cells reached confluency. The amount of IGFBP-4 peptide level in CM correlated for cell number (IGFBP-4 accumulation rate) also rose gradually during the course of culture and plateaued. The IGFBP-4 accumulation rate was strongly negatively correlated with the rate of cell growth (r = 0.98, P < 0.001). In conclusion, our data suggest that in TM-3 cells, cell growth is related to IGFBP-4 accumulation. The negative correlation between IGFBP-4 accumulation and the rate of cell growth suggests that IGFBP-4 may be a primary regulator of TM-3 cell growth and possibly participate in the process of contact inhibition.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalGrowth Hormone and IGF Research
Volume8
Issue number4
Publication statusPublished - 1998

Fingerprint

Insulin-Like Growth Factor Binding Protein 4
Growth
Conditioned Culture Medium
Contact Inhibition
Insulin-Like Growth Factor Binding Proteins
Peptides
Cell Count
Leydig Cells
Somatomedins

Keywords

  • Cell growth
  • Contact inhibition
  • Insulin-like growth factor binding protein-4
  • TM-3 cells

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Insulin-like growth factor binding protein-4 accumulation is negatively correlated with growth rate in TM-3 cells. / Hasegawa, Tomonobu; Hasegawa, Y.; Rosenfeld, R. G.; Cohen, P.

In: Growth Hormone and IGF Research, Vol. 8, No. 4, 1998, p. 277-282.

Research output: Contribution to journalArticle

@article{63a5f207487b45c0a9ac897e5d6e50fc,
title = "Insulin-like growth factor binding protein-4 accumulation is negatively correlated with growth rate in TM-3 cells",
abstract = "Cellular growth is controlled by multiple regulators, including the insulin-like growth factors (IGFs). In some cells, the IGF binding proteins (IGFBPs) are thought to be inhibitory molecules for cell growth and may be related to the process of contact inhibition. In the TM-3 (mouse Leydig) cell line, IGFBP-4 is the major IGFBP secreted into conditioned media (CM), as we have reported. In this study, we investigated cell growth, the peptide levels of IGFBP-4 in CM, and the inverse relationship between IGFBP-4 accumulation and cell growth rate. Quantification of TM-3 growth in serum-containing media demonstrated that TM-3 cell number gradually rose after plating, and plateaued when cells became confluent. The rate of cell growth fell gradually, and net cell growth stopped when cells reached confluency. IGFBP- 4 peptide levels in CM, as measured by Western ligand blot, rose gradually during the culture period and plateaued when cells reached confluency. The amount of IGFBP-4 peptide level in CM correlated for cell number (IGFBP-4 accumulation rate) also rose gradually during the course of culture and plateaued. The IGFBP-4 accumulation rate was strongly negatively correlated with the rate of cell growth (r = 0.98, P < 0.001). In conclusion, our data suggest that in TM-3 cells, cell growth is related to IGFBP-4 accumulation. The negative correlation between IGFBP-4 accumulation and the rate of cell growth suggests that IGFBP-4 may be a primary regulator of TM-3 cell growth and possibly participate in the process of contact inhibition.",
keywords = "Cell growth, Contact inhibition, Insulin-like growth factor binding protein-4, TM-3 cells",
author = "Tomonobu Hasegawa and Y. Hasegawa and Rosenfeld, {R. G.} and P. Cohen",
year = "1998",
language = "English",
volume = "8",
pages = "277--282",
journal = "Endocrinology and Metabolism",
issn = "1096-6374",
publisher = "Churchill Livingstone",
number = "4",

}

TY - JOUR

T1 - Insulin-like growth factor binding protein-4 accumulation is negatively correlated with growth rate in TM-3 cells

AU - Hasegawa, Tomonobu

AU - Hasegawa, Y.

AU - Rosenfeld, R. G.

AU - Cohen, P.

PY - 1998

Y1 - 1998

N2 - Cellular growth is controlled by multiple regulators, including the insulin-like growth factors (IGFs). In some cells, the IGF binding proteins (IGFBPs) are thought to be inhibitory molecules for cell growth and may be related to the process of contact inhibition. In the TM-3 (mouse Leydig) cell line, IGFBP-4 is the major IGFBP secreted into conditioned media (CM), as we have reported. In this study, we investigated cell growth, the peptide levels of IGFBP-4 in CM, and the inverse relationship between IGFBP-4 accumulation and cell growth rate. Quantification of TM-3 growth in serum-containing media demonstrated that TM-3 cell number gradually rose after plating, and plateaued when cells became confluent. The rate of cell growth fell gradually, and net cell growth stopped when cells reached confluency. IGFBP- 4 peptide levels in CM, as measured by Western ligand blot, rose gradually during the culture period and plateaued when cells reached confluency. The amount of IGFBP-4 peptide level in CM correlated for cell number (IGFBP-4 accumulation rate) also rose gradually during the course of culture and plateaued. The IGFBP-4 accumulation rate was strongly negatively correlated with the rate of cell growth (r = 0.98, P < 0.001). In conclusion, our data suggest that in TM-3 cells, cell growth is related to IGFBP-4 accumulation. The negative correlation between IGFBP-4 accumulation and the rate of cell growth suggests that IGFBP-4 may be a primary regulator of TM-3 cell growth and possibly participate in the process of contact inhibition.

AB - Cellular growth is controlled by multiple regulators, including the insulin-like growth factors (IGFs). In some cells, the IGF binding proteins (IGFBPs) are thought to be inhibitory molecules for cell growth and may be related to the process of contact inhibition. In the TM-3 (mouse Leydig) cell line, IGFBP-4 is the major IGFBP secreted into conditioned media (CM), as we have reported. In this study, we investigated cell growth, the peptide levels of IGFBP-4 in CM, and the inverse relationship between IGFBP-4 accumulation and cell growth rate. Quantification of TM-3 growth in serum-containing media demonstrated that TM-3 cell number gradually rose after plating, and plateaued when cells became confluent. The rate of cell growth fell gradually, and net cell growth stopped when cells reached confluency. IGFBP- 4 peptide levels in CM, as measured by Western ligand blot, rose gradually during the culture period and plateaued when cells reached confluency. The amount of IGFBP-4 peptide level in CM correlated for cell number (IGFBP-4 accumulation rate) also rose gradually during the course of culture and plateaued. The IGFBP-4 accumulation rate was strongly negatively correlated with the rate of cell growth (r = 0.98, P < 0.001). In conclusion, our data suggest that in TM-3 cells, cell growth is related to IGFBP-4 accumulation. The negative correlation between IGFBP-4 accumulation and the rate of cell growth suggests that IGFBP-4 may be a primary regulator of TM-3 cell growth and possibly participate in the process of contact inhibition.

KW - Cell growth

KW - Contact inhibition

KW - Insulin-like growth factor binding protein-4

KW - TM-3 cells

UR - http://www.scopus.com/inward/record.url?scp=0032461591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032461591&partnerID=8YFLogxK

M3 - Article

C2 - 10984318

AN - SCOPUS:0032461591

VL - 8

SP - 277

EP - 282

JO - Endocrinology and Metabolism

JF - Endocrinology and Metabolism

SN - 1096-6374

IS - 4

ER -