Insulin receptor and altered glucose transport in a monensin-resistant mutant of Chinese hamster ovary cell

T. Seguchi, Akihiko Yoshimura, M. Ono, S. Shite, M. Kasahara, Y. Ebina, W. J. Rutter, M. Kuwano

Research output: Contribution to journalArticle

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Abstract

A monensin-resistant mutant Mon(r)-31, derived from Chinese hamster ovary (CHO) cell line, has been shown to have a reduced number of insulin receptors and a reduction in glucose uptake in response to insulin. We have further investigated the possibility that altered glucose uptake in Mon(r)-31 cells is related to an alteration in the activity of the insulin receptor. Uptake of glucosamine, 2-deoxy-D-glucose, and 3-O-methyl-D-glucose in Mon(r)-31 cells was one-half to one-third that of CHO cells. The cellular content of the glucose transporter in Mon(r)-31 was reduced to about one-third that of CHO as assayed by use of an antiglucose transporter antibody. After transfection with the human insulin receptor cDNA, we obtained clones CIR-0 from CHO, and MIR-2 and MIR-15 from Mon(r)-31; CIR-0 expressed a tenfold higher level of the insulin-binding activity than did CHO, and MIR-2 and MIR-15 expressed a 20-fold higher level than did Mon(r)-31. Glucose uptake in both CHO and CIR-0 was significantly enhanced by exogenous insulin, but not in Mon(r)-31, MIR-2, and MIR-15. The β-subunits of insulin receptor in CHO, CIR-0, Mon(r)-31, and MIR-2 were similarly phosphorylated. The decreased glucose transport activity in Mon(r)-31 cells is discussed in relation to the absence or presence of insulin receptor expression.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalJournal of Cellular Physiology
Volume139
Issue number2
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Monensin
Insulin Receptor
Cricetulus
Ovary
Cells
Glucose
Insulin
3-O-Methylglucose
Facilitative Glucose Transport Proteins
Glucosamine
Deoxyglucose
Complementary DNA
Antibodies
Transfection
Clone Cells
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Seguchi, T., Yoshimura, A., Ono, M., Shite, S., Kasahara, M., Ebina, Y., ... Kuwano, M. (1989). Insulin receptor and altered glucose transport in a monensin-resistant mutant of Chinese hamster ovary cell. Journal of Cellular Physiology, 139(2), 229-236.

Insulin receptor and altered glucose transport in a monensin-resistant mutant of Chinese hamster ovary cell. / Seguchi, T.; Yoshimura, Akihiko; Ono, M.; Shite, S.; Kasahara, M.; Ebina, Y.; Rutter, W. J.; Kuwano, M.

In: Journal of Cellular Physiology, Vol. 139, No. 2, 1989, p. 229-236.

Research output: Contribution to journalArticle

Seguchi, T, Yoshimura, A, Ono, M, Shite, S, Kasahara, M, Ebina, Y, Rutter, WJ & Kuwano, M 1989, 'Insulin receptor and altered glucose transport in a monensin-resistant mutant of Chinese hamster ovary cell', Journal of Cellular Physiology, vol. 139, no. 2, pp. 229-236.
Seguchi, T. ; Yoshimura, Akihiko ; Ono, M. ; Shite, S. ; Kasahara, M. ; Ebina, Y. ; Rutter, W. J. ; Kuwano, M. / Insulin receptor and altered glucose transport in a monensin-resistant mutant of Chinese hamster ovary cell. In: Journal of Cellular Physiology. 1989 ; Vol. 139, No. 2. pp. 229-236.
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