Intact corneal epithelium is essential for the prevention of stromal haze after laser assisted in situ keratomileusis

K. Nakamura, D. Kurosaka, H. Bissen-Miyajima, Kazuo Tsubota

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Aims - To determine the effect of intact corneal epithelium on stromal haze and myofibroblast cell formation after excimer laser surgery. Methods - Denuded epithelium alone, photorefractive keratectomy (PRK), laser in situ keratomileusis (LASIK), or LASIK with denuded epithelium was performed in rabbit eyes. Postoperative anterior stromal haze was assessed employing a standard scale. Immunohistochemical methods were used to detect alpha smooth muscle actin (α-SMA), a marker for myofibroblastic cells, and type III collagen in subepithelial corneal tissue. Results - Three weeks after surgery, the presence of α-SMA positive long extended and spindle-shaped stromal cells, and synthesis of type III collagen were observed in the subepithelial stromal layer corresponding to corneal haze in PRK and LASIK with denuded epithelium, but not in denuded epithelium alone and LASIK. Conclusion - The intact corneal epithelium may play an important part curbing subepithelial haze and differentiation of myofibroblasts in corneal wound healing.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalBritish Journal of Ophthalmology
Volume85
Issue number2
DOIs
Publication statusPublished - 2001

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Laser In Situ Keratomileusis
Corneal Epithelium
Epithelium
Photorefractive Keratectomy
Collagen Type III
Myofibroblasts
Excimer Lasers
Laser Therapy
Stromal Cells
Wound Healing
Smooth Muscle
Actins
Rabbits

ASJC Scopus subject areas

  • Ophthalmology

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Intact corneal epithelium is essential for the prevention of stromal haze after laser assisted in situ keratomileusis. / Nakamura, K.; Kurosaka, D.; Bissen-Miyajima, H.; Tsubota, Kazuo.

In: British Journal of Ophthalmology, Vol. 85, No. 2, 2001, p. 209-213.

Research output: Contribution to journalArticle

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