Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid

Xiaoguang Li, Hua Qian, Ryosuke Sogame, Yoshiaki Hirako, Daisuke Tsuruta, Norito Ishii, Hiroshi Koga, Atsunari Tsuchisaka, Zhexiong Jin, Kazuo Tsubota, Akiko Fukumoto, Chie Sotozono, Shigeru Kinoshita, Takashi Hashimoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Previous studies of ocular mucous membrane pemphigoid (OMMP) have identified several components of the basement membrane zone to be autoantigens, including integrin β4. However, there are no extensive or definitive reported studies that address this, particularly in pure OMMP. Objectives: To clarify the major autoantigens in pure OMMP. Materials and methods: In this study, we examined sera from 43 pure OMMP patients for both IgG and IgA antibodies using newly developed immunoblotting analyses with a hemidesmosome-rich fraction and various recombinant proteins of integrin α6β4, in addition to our routine immune-serological tests. Results: Using a hemidesmosome-rich fraction, sera from patients with pure OMMP demonstrated reactivity of IgG and/or IgA antibodies to integrin β4, BP180 and laminin- 332. The reactivity of pure OMMP sera to integrin β4 was further confirmed by immunoblotting using integrin β4 recombinant proteins. Using concentrated supernatant of HaCaT cells, only one serum sample showed positive IgG and IgA reactivity to LAD-1, the ectodomain of BP180. None of the pure OMMP sera reacted with any autoantigens on immunoblotting using normal human epidermal or dermal extracts, or purified human laminin-332. Conclusions: Integrin β4was considered to be the major and specific autoantigen for pureOMMP. The new methods established in this study are useful for detection of various autoantigens, particularly integrin β4.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalEuropean Journal of Dermatology
Volume26
Issue number3
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

Bullous Pemphigoid
Integrins
Mucous Membrane
Antigens
Autoantigens
Immunoblotting
Hemidesmosomes
Immunoglobulin A
Serum
Immunoglobulin G
Recombinant Proteins
Antibodies
Serologic Tests
Basement Membrane
Skin

Keywords

  • Autoantibody
  • Autoantigen
  • Hemidesmosome-rich fraction
  • Integrin α6β4
  • Pure ocular mucous membrane pemphigoid

ASJC Scopus subject areas

  • Dermatology

Cite this

Li, X., Qian, H., Sogame, R., Hirako, Y., Tsuruta, D., Ishii, N., ... Hashimoto, T. (2016). Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid. European Journal of Dermatology, 26(3), 247-253. https://doi.org/10.1684/ejd.2016.2772

Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid. / Li, Xiaoguang; Qian, Hua; Sogame, Ryosuke; Hirako, Yoshiaki; Tsuruta, Daisuke; Ishii, Norito; Koga, Hiroshi; Tsuchisaka, Atsunari; Jin, Zhexiong; Tsubota, Kazuo; Fukumoto, Akiko; Sotozono, Chie; Kinoshita, Shigeru; Hashimoto, Takashi.

In: European Journal of Dermatology, Vol. 26, No. 3, 01.05.2016, p. 247-253.

Research output: Contribution to journalArticle

Li, X, Qian, H, Sogame, R, Hirako, Y, Tsuruta, D, Ishii, N, Koga, H, Tsuchisaka, A, Jin, Z, Tsubota, K, Fukumoto, A, Sotozono, C, Kinoshita, S & Hashimoto, T 2016, 'Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid', European Journal of Dermatology, vol. 26, no. 3, pp. 247-253. https://doi.org/10.1684/ejd.2016.2772
Li, Xiaoguang ; Qian, Hua ; Sogame, Ryosuke ; Hirako, Yoshiaki ; Tsuruta, Daisuke ; Ishii, Norito ; Koga, Hiroshi ; Tsuchisaka, Atsunari ; Jin, Zhexiong ; Tsubota, Kazuo ; Fukumoto, Akiko ; Sotozono, Chie ; Kinoshita, Shigeru ; Hashimoto, Takashi. / Integrin β4 is a major target antigen in pure ocular mucous membrane pemphigoid. In: European Journal of Dermatology. 2016 ; Vol. 26, No. 3. pp. 247-253.
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AU - Li, Xiaoguang

AU - Qian, Hua

AU - Sogame, Ryosuke

AU - Hirako, Yoshiaki

AU - Tsuruta, Daisuke

AU - Ishii, Norito

AU - Koga, Hiroshi

AU - Tsuchisaka, Atsunari

AU - Jin, Zhexiong

AU - Tsubota, Kazuo

AU - Fukumoto, Akiko

AU - Sotozono, Chie

AU - Kinoshita, Shigeru

AU - Hashimoto, Takashi

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N2 - Background: Previous studies of ocular mucous membrane pemphigoid (OMMP) have identified several components of the basement membrane zone to be autoantigens, including integrin β4. However, there are no extensive or definitive reported studies that address this, particularly in pure OMMP. Objectives: To clarify the major autoantigens in pure OMMP. Materials and methods: In this study, we examined sera from 43 pure OMMP patients for both IgG and IgA antibodies using newly developed immunoblotting analyses with a hemidesmosome-rich fraction and various recombinant proteins of integrin α6β4, in addition to our routine immune-serological tests. Results: Using a hemidesmosome-rich fraction, sera from patients with pure OMMP demonstrated reactivity of IgG and/or IgA antibodies to integrin β4, BP180 and laminin- 332. The reactivity of pure OMMP sera to integrin β4 was further confirmed by immunoblotting using integrin β4 recombinant proteins. Using concentrated supernatant of HaCaT cells, only one serum sample showed positive IgG and IgA reactivity to LAD-1, the ectodomain of BP180. None of the pure OMMP sera reacted with any autoantigens on immunoblotting using normal human epidermal or dermal extracts, or purified human laminin-332. Conclusions: Integrin β4was considered to be the major and specific autoantigen for pureOMMP. The new methods established in this study are useful for detection of various autoantigens, particularly integrin β4.

AB - Background: Previous studies of ocular mucous membrane pemphigoid (OMMP) have identified several components of the basement membrane zone to be autoantigens, including integrin β4. However, there are no extensive or definitive reported studies that address this, particularly in pure OMMP. Objectives: To clarify the major autoantigens in pure OMMP. Materials and methods: In this study, we examined sera from 43 pure OMMP patients for both IgG and IgA antibodies using newly developed immunoblotting analyses with a hemidesmosome-rich fraction and various recombinant proteins of integrin α6β4, in addition to our routine immune-serological tests. Results: Using a hemidesmosome-rich fraction, sera from patients with pure OMMP demonstrated reactivity of IgG and/or IgA antibodies to integrin β4, BP180 and laminin- 332. The reactivity of pure OMMP sera to integrin β4 was further confirmed by immunoblotting using integrin β4 recombinant proteins. Using concentrated supernatant of HaCaT cells, only one serum sample showed positive IgG and IgA reactivity to LAD-1, the ectodomain of BP180. None of the pure OMMP sera reacted with any autoantigens on immunoblotting using normal human epidermal or dermal extracts, or purified human laminin-332. Conclusions: Integrin β4was considered to be the major and specific autoantigen for pureOMMP. The new methods established in this study are useful for detection of various autoantigens, particularly integrin β4.

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