Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume45
Issue number29-32
DOIs
Publication statusPublished - 2006 Aug 11

Fingerprint

stress waves
plasmids
genes
integrity
DNA
deoxyribonucleic acid
Genes
Lasers
lasers
Electrophoresis
Laser pulses
electrophoresis
Irradiation
buffers
irradiation
pulses
Experiments

Keywords

  • DNA integrity
  • Electrophoresis
  • Gene therapy
  • Gene transfection
  • Heating
  • Laser-induced stress waves

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection. / Terakawa, Mitsuhiro; Sato, Shunichi; Ashida, Hiroshi; Okano, Hideyuki; Obara, Minoru.

In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 45, No. 29-32, 11.08.2006.

Research output: Contribution to journalArticle

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