Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

Mitsuhiro Terakawa; Shunichi Sato; Hiroshi Ashida; Hideyuki Okano; Minoru Obara

doi:10.1143/JJAP.45.L768

Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.

Original language	English
Pages (from-to)	L768-L769
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	45
Issue number	29-32
DOIs	https://doi.org/10.1143/JJAP.45.L768
Publication status	Published - 2006 Aug 11

Keywords

DNA integrity
Electrophoresis
Gene therapy
Gene transfection
Heating
Laser-induced stress waves

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

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10.1143/JJAP.45.L768

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abstract = "We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.",

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AU - Terakawa, Mitsuhiro

AU - Sato, Shunichi

AU - Ashida, Hiroshi

AU - Okano, Hideyuki

AU - Obara, Minoru

PY - 2006/8/11

Y1 - 2006/8/11

N2 - We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.

AB - We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.

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KW - Gene therapy

KW - Gene transfection

KW - Heating

KW - Laser-induced stress waves

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JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

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Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

Abstract

Keywords

ASJC Scopus subject areas

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