Interactions between kisspeptin neurons and hypothalamic tuberoinfundibular dopaminergic neurons in aged female rats

Kinuyo Iwata, Masaaki Ikehara, Yuyu Kunimura, Hitoshi Ozawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Kisspeptin neurons in the arcuate nucleus (ARC) regulate prolactin secretion, and are in physical contact with tuberoinfundibular dopaminergic (TIDA) neurons, which inhibit prolactin secretion. Prolactin levels in the blood are increased with advancing age in rats; therefore, we investigated the interactions with TIDA neurons and kisspeptin neurons in aged female rats (24 months of age), relative to those of young adult female rats (9-10 weeks of age). Plasma prolactin levels in the aged rats were significantly higher than those of young adult rats. Tyrosine hydroxylase (TH)-immunoreactive (ir) cell bodies and kisspeptin-ir nerve fibers were found in the dorsomedial ARC of both groups. The number of TH-ir cell bodies in the dorsomedial ARC did not differ significantly between groups. Additionally, no significant differences in the number of TH-ir cells in contact with kisspeptin-ir fibers was observed between groups. However, the number of kisspeptin-ir or Kiss1 mRNA-expressing cells in the ARC was significantly reduced in the aged rats compared with that of the young rats. These results suggest that the contacts between TIDA neurons and kisspeptin neurons are maintained after reproductive senescence, while production of kisspeptin in the ARC decreases significantly during aging.

Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalActa Histochemica et Cytochemica
Volume49
Issue number6
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint

Kisspeptins
Dopaminergic Neurons
Neurons
Arcuate Nucleus of Hypothalamus
Rats
Prolactin
Tyrosine 3-Monooxygenase
Mediodorsal Thalamic Nucleus
Young Adult
Cells
Fibers
Nerve Fibers
Blood
Aging of materials
Plasmas
Messenger RNA

Keywords

  • Aging
  • Dopamine
  • Kisspeptin
  • Prolactin
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

Cite this

Interactions between kisspeptin neurons and hypothalamic tuberoinfundibular dopaminergic neurons in aged female rats. / Iwata, Kinuyo; Ikehara, Masaaki; Kunimura, Yuyu; Ozawa, Hitoshi.

In: Acta Histochemica et Cytochemica, Vol. 49, No. 6, 2016, p. 191-196.

Research output: Contribution to journalArticle

@article{b2ba56c5715d4e6ea72cf637404b4cdd,
title = "Interactions between kisspeptin neurons and hypothalamic tuberoinfundibular dopaminergic neurons in aged female rats",
abstract = "Kisspeptin neurons in the arcuate nucleus (ARC) regulate prolactin secretion, and are in physical contact with tuberoinfundibular dopaminergic (TIDA) neurons, which inhibit prolactin secretion. Prolactin levels in the blood are increased with advancing age in rats; therefore, we investigated the interactions with TIDA neurons and kisspeptin neurons in aged female rats (24 months of age), relative to those of young adult female rats (9-10 weeks of age). Plasma prolactin levels in the aged rats were significantly higher than those of young adult rats. Tyrosine hydroxylase (TH)-immunoreactive (ir) cell bodies and kisspeptin-ir nerve fibers were found in the dorsomedial ARC of both groups. The number of TH-ir cell bodies in the dorsomedial ARC did not differ significantly between groups. Additionally, no significant differences in the number of TH-ir cells in contact with kisspeptin-ir fibers was observed between groups. However, the number of kisspeptin-ir or Kiss1 mRNA-expressing cells in the ARC was significantly reduced in the aged rats compared with that of the young rats. These results suggest that the contacts between TIDA neurons and kisspeptin neurons are maintained after reproductive senescence, while production of kisspeptin in the ARC decreases significantly during aging.",
keywords = "Aging, Dopamine, Kisspeptin, Prolactin, Tyrosine hydroxylase",
author = "Kinuyo Iwata and Masaaki Ikehara and Yuyu Kunimura and Hitoshi Ozawa",
year = "2016",
doi = "10.1267/ahc.16027",
language = "English",
volume = "49",
pages = "191--196",
journal = "Acta Histochemica et Cytochemica",
issn = "0044-5991",
publisher = "Japan Society of Histochemistry and Cytochemistry",
number = "6",

}

TY - JOUR

T1 - Interactions between kisspeptin neurons and hypothalamic tuberoinfundibular dopaminergic neurons in aged female rats

AU - Iwata, Kinuyo

AU - Ikehara, Masaaki

AU - Kunimura, Yuyu

AU - Ozawa, Hitoshi

PY - 2016

Y1 - 2016

N2 - Kisspeptin neurons in the arcuate nucleus (ARC) regulate prolactin secretion, and are in physical contact with tuberoinfundibular dopaminergic (TIDA) neurons, which inhibit prolactin secretion. Prolactin levels in the blood are increased with advancing age in rats; therefore, we investigated the interactions with TIDA neurons and kisspeptin neurons in aged female rats (24 months of age), relative to those of young adult female rats (9-10 weeks of age). Plasma prolactin levels in the aged rats were significantly higher than those of young adult rats. Tyrosine hydroxylase (TH)-immunoreactive (ir) cell bodies and kisspeptin-ir nerve fibers were found in the dorsomedial ARC of both groups. The number of TH-ir cell bodies in the dorsomedial ARC did not differ significantly between groups. Additionally, no significant differences in the number of TH-ir cells in contact with kisspeptin-ir fibers was observed between groups. However, the number of kisspeptin-ir or Kiss1 mRNA-expressing cells in the ARC was significantly reduced in the aged rats compared with that of the young rats. These results suggest that the contacts between TIDA neurons and kisspeptin neurons are maintained after reproductive senescence, while production of kisspeptin in the ARC decreases significantly during aging.

AB - Kisspeptin neurons in the arcuate nucleus (ARC) regulate prolactin secretion, and are in physical contact with tuberoinfundibular dopaminergic (TIDA) neurons, which inhibit prolactin secretion. Prolactin levels in the blood are increased with advancing age in rats; therefore, we investigated the interactions with TIDA neurons and kisspeptin neurons in aged female rats (24 months of age), relative to those of young adult female rats (9-10 weeks of age). Plasma prolactin levels in the aged rats were significantly higher than those of young adult rats. Tyrosine hydroxylase (TH)-immunoreactive (ir) cell bodies and kisspeptin-ir nerve fibers were found in the dorsomedial ARC of both groups. The number of TH-ir cell bodies in the dorsomedial ARC did not differ significantly between groups. Additionally, no significant differences in the number of TH-ir cells in contact with kisspeptin-ir fibers was observed between groups. However, the number of kisspeptin-ir or Kiss1 mRNA-expressing cells in the ARC was significantly reduced in the aged rats compared with that of the young rats. These results suggest that the contacts between TIDA neurons and kisspeptin neurons are maintained after reproductive senescence, while production of kisspeptin in the ARC decreases significantly during aging.

KW - Aging

KW - Dopamine

KW - Kisspeptin

KW - Prolactin

KW - Tyrosine hydroxylase

UR - http://www.scopus.com/inward/record.url?scp=85007518114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007518114&partnerID=8YFLogxK

U2 - 10.1267/ahc.16027

DO - 10.1267/ahc.16027

M3 - Article

VL - 49

SP - 191

EP - 196

JO - Acta Histochemica et Cytochemica

JF - Acta Histochemica et Cytochemica

SN - 0044-5991

IS - 6

ER -