Interactions between thermosensitive hydrogel microspheres and proteins

Keiji Fujimoto, Yoshio Mizuhara, Nobuko Tamura, Haruma Kawaguchi

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) (PNIPAM) hydrogel microspheres show volume transition based on the LCST around 32°C. It is thought that the surface of the microspheres becomes hydrophobic due to association of segments induced by the hydrophobic interaction above the LCST. Protein adsorption and desorption are dependent both on surface properties of microspheres and on characteristics of protein molecules. In this study, we reported adsorption and desorption of proteins with similar molecular size and shape on poly(styrene) (PSt) and PNIPAM microspheres. Proteins used for this study were myoglobin (MG), α-lactalbumin (LA), lysozyme (LZ), and ribonuclease A (RNase). In addition, peroxidase was used to evaluate the extent of the denaturation induced by adsorption and desorption, and its residual enzyme activity was measured. PNIPAM showed the temperature dependence of protein adsorption, especially in the adsorption of flexible proteins such as LA and MG. It seemed probable that structure rearrangement resulted in the entropy gain. The desorption behavior was also related to the protein's flexibility. It was confirmed from the residual activity that peroxidase desorbed from PNIPAM microspheres remained more stable than peroxidase desorbed from PSt. These hydrogel microspheres were found to be the potent thermosensitive carrier or adsorbent for proteins.

Original languageEnglish
Pages (from-to)184-189
Number of pages6
JournalJournal of Intelligent Material Systems and Structures
Volume4
Issue number2
Publication statusPublished - 1993 Apr

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Hydrogel
Microspheres
Hydrogels
Proteins
Desorption
Adsorption
Peroxidase
Lactalbumin
Myoglobin
Pancreatic Ribonuclease
Denaturation
Styrene
Enzyme activity
Muramidase
Adsorbents
Surface properties
Entropy
Enzymes
Association reactions
Molecules

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Interactions between thermosensitive hydrogel microspheres and proteins. / Fujimoto, Keiji; Mizuhara, Yoshio; Tamura, Nobuko; Kawaguchi, Haruma.

In: Journal of Intelligent Material Systems and Structures, Vol. 4, No. 2, 04.1993, p. 184-189.

Research output: Contribution to journalArticle

Fujimoto, Keiji ; Mizuhara, Yoshio ; Tamura, Nobuko ; Kawaguchi, Haruma. / Interactions between thermosensitive hydrogel microspheres and proteins. In: Journal of Intelligent Material Systems and Structures. 1993 ; Vol. 4, No. 2. pp. 184-189.
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