Interactions of lecithinized superoxide dismutase with serum proteins and cells

Tsutomu Ishihara, Shunsuke Nara, Tohru Mizushima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Superoxide dismutase covalently bound to four lecithin molecules (PC-SOD) is known to be retained in circulating blood for a prolonged period and has a high affinity for cells, resulting in beneficial therapeutic effects in animal disease models. In this study, we evaluated the interaction of PC-SOD with biological components, such as serum proteins and cells, to clarify the mechanism underlying the improved pharmacokinetics of SOD induced by lecithin chemical modification (lecithinization). PC-SOD was distributed in the plasma but not in blood cells after being added to the blood. PC-SOD formed a complex with serum protein(s) such as albumin, whereas unmodified SOD did not. The cellular content of PC-SOD was markedly higher than that of unmodified SOD, and was distributed in lysosomes. The pathway associated with the cellular uptake was found to involve clathrin-/caveolae-independent and cholesterol-sensitive endocytosis. Overall, our data indicated that the increased hydrophobicity of lecithinized SOD enhanced its association to both serum protein(s) and plasma membrane microdomains. The former inhibited SOD excretion and promoted long-term retention in circulating blood, whereas the latter enhanced internalization into cells via endocytosis.

Original languageEnglish
Pages (from-to)1987-1994
Number of pages8
JournalJournal of Pharmaceutical Sciences
Volume103
Issue number7
DOIs
Publication statusPublished - 2014 Jul

Keywords

  • conjugation
  • drug delivery systems
  • macromolecular drug delivery
  • membrane transport
  • pharmacokinetics
  • protein delivery
  • proteins

ASJC Scopus subject areas

  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Interactions of lecithinized superoxide dismutase with serum proteins and cells'. Together they form a unique fingerprint.

  • Cite this