Interleukin-1 receptor immunoreactivity in sympathetic vascular and non-vascular neurons in guinea-pig coeliac ganglion

Judy L. Morris, Phillip Jobling, Toshihiko Shimizu, Ian L. Gibbins

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Immunoreactivity (IR) for the interleukin-1 receptor type I (IL1RI) was examined in sympathetic neurons in guinea-pig coeliac ganglion using multiple-labelling immunofluorescence. IL1RI-IR was present in 8% of sympathetic neurons in untreated preparations. The proportion of neurons with IL1RI-IR increased significantly after incubation in interleukin-6 (200 ng/ml) for 2-4 h (16-26% neurons), or after incubation for 4 h without cytokine (16%), with interleukin-1β (IL1β, 200 ng/ml; 18%) or tumour necrosis factor-α (200 ng/ml; 16%). This increase occurred predominantly in neuropeptide Y-IR, vasoconstrictor neurons. IL1RI-IR also was present in varicose axons, some of which projected from the gut, and in vascular smooth muscle cells and endothelium. These potential binding sites for the proinflammatory cytokine, IL1β, on vasoconstrictor neurons and blood vessels may modulate sympathetic regulation of intestinal blood flow in inflammatory conditions.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalNeuroscience Letters
Volume333
Issue number1
DOIs
Publication statusPublished - 2002 Nov 15
Externally publishedYes

Fingerprint

Sympathetic Ganglia
Interleukin-1 Receptors
Interleukin-1 Type I Receptors
Blood Vessels
Guinea Pigs
Neurons
Vasoconstrictor Agents
Cytokines
Neuropeptide Y
Interleukin-1
Vascular Smooth Muscle
Smooth Muscle Myocytes
Endothelium
Fluorescent Antibody Technique
Axons
Interleukin-6
Tumor Necrosis Factor-alpha
Binding Sites

Keywords

  • Interleukin-1 receptor type I
  • Interleukin-1β
  • Interleukin-6
  • Mesenteric circulation
  • Proinflammatory cytokines
  • Sympathetic neurons
  • Tumour necrosis factor-α

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Interleukin-1 receptor immunoreactivity in sympathetic vascular and non-vascular neurons in guinea-pig coeliac ganglion. / Morris, Judy L.; Jobling, Phillip; Shimizu, Toshihiko; Gibbins, Ian L.

In: Neuroscience Letters, Vol. 333, No. 1, 15.11.2002, p. 54-58.

Research output: Contribution to journalArticle

Morris, Judy L. ; Jobling, Phillip ; Shimizu, Toshihiko ; Gibbins, Ian L. / Interleukin-1 receptor immunoreactivity in sympathetic vascular and non-vascular neurons in guinea-pig coeliac ganglion. In: Neuroscience Letters. 2002 ; Vol. 333, No. 1. pp. 54-58.
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