Interleukin-10 and cAMP-elevating agents cooperate to induce suppressor of cytokine signaling-3 via a protein kinase A-independent signal

Sara Gasperini, Luca Crepaldi, Federica Calzetti, Luana Gatto, Chiara Berlato, Flavia Bazzoni, Akihiko Yoshimura, Marco A. Cassatella

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We have recently shown that IL-10 represents an efficient stimulus for suppressor of cytokine signalling (SOCS)-3 mRNA expression in human neutrophils and PBMC. Herein, we identify cAMP-elevating agents such as prostaglandin E2 (PGE2), PGE1, forskolin, dibutyryl cAMP (dbcAMP) and cholera toxin as a novel class of agonists able to induce SOCS-3 mRNA and protein expression in human leukocytes, cooperating with interleukin-10 (IL-10) in such activities. While PGE2 or dbcAMP prolonged the stability of SOCS-3 mRNA isolated from IL-10-treated leukocytes, inhibitors of cAMP-dependent protein kinase A (H89, KT5720, and St-Ht31 peptide) did not influence the action of PGE2/dbcAMP and/or IL-10 on SOCS-3 mRNA and protein expression, implying that their effect are mediated through a PKA-independent pathway. Taken together, our data identify cAMP-elevating substances as a novel class of agonists able to trigger SOCS-3 expression, and suggest that SOCS-3 might be involved in the regulatory effects of cAMP-elevating substances.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalEuropean Cytokine Network
Volume13
Issue number1
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Cyclic AMP-Dependent Protein Kinases
Interleukin-10
Dinoprostone
Cytokines
Messenger RNA
Leukocytes
Alprostadil
Cholera Toxin
Colforsin
Neutrophils
Peptides
Proteins
Suppressor of Cytokine Signaling 3 Protein

Keywords

  • IL-10
  • Neutrophils
  • PBMC
  • PGE
  • PKA
  • SOCS-3

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Gasperini, S., Crepaldi, L., Calzetti, F., Gatto, L., Berlato, C., Bazzoni, F., ... Cassatella, M. A. (2002). Interleukin-10 and cAMP-elevating agents cooperate to induce suppressor of cytokine signaling-3 via a protein kinase A-independent signal. European Cytokine Network, 13(1), 47-53.

Interleukin-10 and cAMP-elevating agents cooperate to induce suppressor of cytokine signaling-3 via a protein kinase A-independent signal. / Gasperini, Sara; Crepaldi, Luca; Calzetti, Federica; Gatto, Luana; Berlato, Chiara; Bazzoni, Flavia; Yoshimura, Akihiko; Cassatella, Marco A.

In: European Cytokine Network, Vol. 13, No. 1, 2002, p. 47-53.

Research output: Contribution to journalArticle

Gasperini, S, Crepaldi, L, Calzetti, F, Gatto, L, Berlato, C, Bazzoni, F, Yoshimura, A & Cassatella, MA 2002, 'Interleukin-10 and cAMP-elevating agents cooperate to induce suppressor of cytokine signaling-3 via a protein kinase A-independent signal', European Cytokine Network, vol. 13, no. 1, pp. 47-53.
Gasperini, Sara ; Crepaldi, Luca ; Calzetti, Federica ; Gatto, Luana ; Berlato, Chiara ; Bazzoni, Flavia ; Yoshimura, Akihiko ; Cassatella, Marco A. / Interleukin-10 and cAMP-elevating agents cooperate to induce suppressor of cytokine signaling-3 via a protein kinase A-independent signal. In: European Cytokine Network. 2002 ; Vol. 13, No. 1. pp. 47-53.
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