Interleukin-10-induced CCR5 expression in macrophage like HL-60 cells: Involvement of Erk1/2 and STAT-3

Yoko Makuta, Yoshiko Sonoda, Daisuke Yamamoto, Megumi Tago, Eriko Yokota, Yutaka Takebe, Tadashi Kasahara

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

As an immunosuppressive and anti-inflammatory cytokine, IL-10 was recently reported to play roles in CCR5 expression in human monocytes. CCR5 promoter regions contain Oct-2, TCF-1γ, GATA, and STAT binding sites. Here, we studied the signals involved in the CCR5 expression in IL-10-stimulated cells using the HL-60 cell line. HL-60 cells were stimulated with PMA and differentiated to macrophage-like cells, then stimulated with IL-10. IL-10 induced significant expression of CCR5 protein and CCR5 mRNA in these cells. The induction of CCR5 by IL-10 was inhibited by a MEK-1 inhibitor, PD98059. In addition, IL-10 induced tyrosine (Tyr) phosphorylation of Erk, as well as serine (Ser) and Tyr phosphorylation of STAT-3. Tyr phosphorylation of Erk and Ser phosphorylation of STAT-3 were inhibited by PD98059, while Tyr phosphorylation of STAT-3 was not inhibited by PD98059. DNA binding activity of STAT-3 was observed by the stimulation with IL-10, which was inhibited by PD98059. These results first indicate that Erk1/2 and STAT-3 regulate CCR5 expression, and that Erk-mediated phosphorylation of Ser is required for full stimulation of STAT-3 in CCR5 expression.

Original languageEnglish
Pages (from-to)1076-1081
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume26
Issue number8
Publication statusPublished - 2003 Aug

Fingerprint

HL-60 Cells
Interleukin-10
Macrophages
Phosphorylation
Tyrosine
Serine
Mitogen-Activated Protein Kinase Kinases
Immunosuppressive Agents
Genetic Promoter Regions
Monocytes
Anti-Inflammatory Agents
Binding Sites
Cytokines
Cell Line
Messenger RNA
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
DNA
Proteins

Keywords

  • CCR5
  • IL-10
  • MEK inhibitor
  • STAT-3

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Interleukin-10-induced CCR5 expression in macrophage like HL-60 cells : Involvement of Erk1/2 and STAT-3. / Makuta, Yoko; Sonoda, Yoshiko; Yamamoto, Daisuke; Tago, Megumi; Yokota, Eriko; Takebe, Yutaka; Kasahara, Tadashi.

In: Biological and Pharmaceutical Bulletin, Vol. 26, No. 8, 08.2003, p. 1076-1081.

Research output: Contribution to journalArticle

Makuta, Yoko ; Sonoda, Yoshiko ; Yamamoto, Daisuke ; Tago, Megumi ; Yokota, Eriko ; Takebe, Yutaka ; Kasahara, Tadashi. / Interleukin-10-induced CCR5 expression in macrophage like HL-60 cells : Involvement of Erk1/2 and STAT-3. In: Biological and Pharmaceutical Bulletin. 2003 ; Vol. 26, No. 8. pp. 1076-1081.
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