Interleukin-13 selectively suppresses the growth of human macrophage progenitors at the late stage

O. Sakamoto, M. Hashiyama, A. Minty, M. Ando, T. Suda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Interleukin-13 (IL-13) is a pleiotropic cytokine that inhibits the production of inflammatory cytokines of monocytes. We investigated the effects of IL-13 on the clonal growth of human hematopoietic progenitors, IL- 13 alone did not support any colony formation. IL-13 markedly suppressed macrophage colonies that were formed in the presence of IL-3 and erythropoietin, granulocyte-macrophage colony-stimulating factor, or macrophage colony-stimulating factor. Macrophage colony cells showed dendritic cell-like morphology and cellular aggregates. IL-13 did not affect granulocyte colony and erythroid burst formation. Delayed addition of IL-13 and replating onto the culture dishes with IL-13 showed that macrophage colony formation was suppressed during days 8 and 14 of culture. These results indicate that IL-13 affects the growth of the late stage of committed macrophage progenitors. Single-cell culture of isolated CD34+CD33+ cells with IL-13 confirmed that macrophage colony formation was significantly suppressed. These results show that IL-13 directly suppresses the proliferation of differentiating macrophages. In addition, these suppressive effects of IL-13 were synergistic with IL-4. Furthermore, in the liquid culture of bone marrow cells in the presence of IL-13, the number of CD14 (monocyte-macrophage antigen)positive cells decreased and CD18 (LFA-1β)- positive cells increased. It is concluded that IL-13 affects the growth of the late stage of macrophage precursors as well as mature monocytes. Induction of differentiation of human monocytes maybe correlated with the suppression of their progenitors.

Original languageEnglish
Pages (from-to)3487-3493
Number of pages7
JournalBlood
Volume85
Issue number12
Publication statusPublished - 1995

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Interleukin-13
Macrophages
Growth
Monocytes
Cell culture
Cells
Cytokines
Lymphocyte Function-Associated Antigen-1
Macrophage Colony-Stimulating Factor
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Granulocytes
Bone Marrow Cells
Interleukin-4
Dendritic Cells
Bone
Cell Culture Techniques

ASJC Scopus subject areas

  • Hematology

Cite this

Sakamoto, O., Hashiyama, M., Minty, A., Ando, M., & Suda, T. (1995). Interleukin-13 selectively suppresses the growth of human macrophage progenitors at the late stage. Blood, 85(12), 3487-3493.

Interleukin-13 selectively suppresses the growth of human macrophage progenitors at the late stage. / Sakamoto, O.; Hashiyama, M.; Minty, A.; Ando, M.; Suda, T.

In: Blood, Vol. 85, No. 12, 1995, p. 3487-3493.

Research output: Contribution to journalArticle

Sakamoto, O, Hashiyama, M, Minty, A, Ando, M & Suda, T 1995, 'Interleukin-13 selectively suppresses the growth of human macrophage progenitors at the late stage', Blood, vol. 85, no. 12, pp. 3487-3493.
Sakamoto O, Hashiyama M, Minty A, Ando M, Suda T. Interleukin-13 selectively suppresses the growth of human macrophage progenitors at the late stage. Blood. 1995;85(12):3487-3493.
Sakamoto, O. ; Hashiyama, M. ; Minty, A. ; Ando, M. ; Suda, T. / Interleukin-13 selectively suppresses the growth of human macrophage progenitors at the late stage. In: Blood. 1995 ; Vol. 85, No. 12. pp. 3487-3493.
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