Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts

Akira Andoh, Hirofumi Yasui, Osamu Inatomi, Zhuobin Zhang, Yasuyuki Deguchi, Kazunori Hata, Yoshio Araki, Tomoyuki Tsujikawa, Katsuyuki Kitoh, Shokei Kim-Mitsuyama, Atsushi Takayanagi, Nobuyoshi Shimizu, Yoshihide Fujiyama

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background. Interleukin (IL)-17 is a newly identified T-cell-specific cytokine. In this study, we investigated the effects of IL-17 on colony-stimulating factor (CSF) release in human colonic subepithelial myofibroblasts (SEMFs). Methods. CSF release and mRNA expression were determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting, respectively. Nuclear factor (NF)-κB- and activating protein (AP-1)-DNA binding activities were evaluated by electrophoretic gel mobility shift assays (EMSAs). Results. Unstimulated cells secreted as mall amount of granulocyte G- and granulocyte/macrophage (GM)-CSF, and a considerable amount of M-CSF. IL-17 weakly enhanced G-CSF release, but did not affect GM- and M-CSF release. IL-17 selectively enhanced tumor necrosis factor (TNF)-α-induced G- and GM-CSF release. The combination of IL-17 plus TNF-α induced a marked increase in NF-κB- and AP-1-DNA binding activities. The adenovirus-mediated transfer of a stable form of IκBα and/or a dominant negative mutant of c-Jun markedly inhibited the IL-17 plus TNF-α-induced G- and GM-CSF mRNA expression. Furthermore, a stability study showed that IL-17 plus TNF-α markedly enhanced the stability of G- and GM-CSF mRNA. Conclusions. IL-17 augments TNF-α-induced G- and GM-CSF release via transcriptional and posttranscriptional mechanisms.

Original languageEnglish
Pages (from-to)802-810
Number of pages9
JournalJournal of Gastroenterology
Volume40
Issue number8
DOIs
Publication statusPublished - 2005 Aug

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Myofibroblasts
Interleukin-17
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocytes
Tumor Necrosis Factor-alpha
Colony-Stimulating Factors
Transcription Factor AP-1
Messenger RNA
DNA
Electrophoretic Mobility Shift Assay
Adenoviridae
Northern Blotting
Gels
Enzyme-Linked Immunosorbent Assay
Cytokines
T-Lymphocytes

Keywords

  • AP-1
  • M-CSF
  • NF-κB
  • T cells

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts. / Andoh, Akira; Yasui, Hirofumi; Inatomi, Osamu; Zhang, Zhuobin; Deguchi, Yasuyuki; Hata, Kazunori; Araki, Yoshio; Tsujikawa, Tomoyuki; Kitoh, Katsuyuki; Kim-Mitsuyama, Shokei; Takayanagi, Atsushi; Shimizu, Nobuyoshi; Fujiyama, Yoshihide.

In: Journal of Gastroenterology, Vol. 40, No. 8, 08.2005, p. 802-810.

Research output: Contribution to journalArticle

Andoh, A, Yasui, H, Inatomi, O, Zhang, Z, Deguchi, Y, Hata, K, Araki, Y, Tsujikawa, T, Kitoh, K, Kim-Mitsuyama, S, Takayanagi, A, Shimizu, N & Fujiyama, Y 2005, 'Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts', Journal of Gastroenterology, vol. 40, no. 8, pp. 802-810. https://doi.org/10.1007/s00535-005-1632-x
Andoh, Akira ; Yasui, Hirofumi ; Inatomi, Osamu ; Zhang, Zhuobin ; Deguchi, Yasuyuki ; Hata, Kazunori ; Araki, Yoshio ; Tsujikawa, Tomoyuki ; Kitoh, Katsuyuki ; Kim-Mitsuyama, Shokei ; Takayanagi, Atsushi ; Shimizu, Nobuyoshi ; Fujiyama, Yoshihide. / Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts. In: Journal of Gastroenterology. 2005 ; Vol. 40, No. 8. pp. 802-810.
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title = "Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts",
abstract = "Background. Interleukin (IL)-17 is a newly identified T-cell-specific cytokine. In this study, we investigated the effects of IL-17 on colony-stimulating factor (CSF) release in human colonic subepithelial myofibroblasts (SEMFs). Methods. CSF release and mRNA expression were determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting, respectively. Nuclear factor (NF)-κB- and activating protein (AP-1)-DNA binding activities were evaluated by electrophoretic gel mobility shift assays (EMSAs). Results. Unstimulated cells secreted as mall amount of granulocyte G- and granulocyte/macrophage (GM)-CSF, and a considerable amount of M-CSF. IL-17 weakly enhanced G-CSF release, but did not affect GM- and M-CSF release. IL-17 selectively enhanced tumor necrosis factor (TNF)-α-induced G- and GM-CSF release. The combination of IL-17 plus TNF-α induced a marked increase in NF-κB- and AP-1-DNA binding activities. The adenovirus-mediated transfer of a stable form of IκBα and/or a dominant negative mutant of c-Jun markedly inhibited the IL-17 plus TNF-α-induced G- and GM-CSF mRNA expression. Furthermore, a stability study showed that IL-17 plus TNF-α markedly enhanced the stability of G- and GM-CSF mRNA. Conclusions. IL-17 augments TNF-α-induced G- and GM-CSF release via transcriptional and posttranscriptional mechanisms.",
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author = "Akira Andoh and Hirofumi Yasui and Osamu Inatomi and Zhuobin Zhang and Yasuyuki Deguchi and Kazunori Hata and Yoshio Araki and Tomoyuki Tsujikawa and Katsuyuki Kitoh and Shokei Kim-Mitsuyama and Atsushi Takayanagi and Nobuyoshi Shimizu and Yoshihide Fujiyama",
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T1 - Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts

AU - Andoh, Akira

AU - Yasui, Hirofumi

AU - Inatomi, Osamu

AU - Zhang, Zhuobin

AU - Deguchi, Yasuyuki

AU - Hata, Kazunori

AU - Araki, Yoshio

AU - Tsujikawa, Tomoyuki

AU - Kitoh, Katsuyuki

AU - Kim-Mitsuyama, Shokei

AU - Takayanagi, Atsushi

AU - Shimizu, Nobuyoshi

AU - Fujiyama, Yoshihide

PY - 2005/8

Y1 - 2005/8

N2 - Background. Interleukin (IL)-17 is a newly identified T-cell-specific cytokine. In this study, we investigated the effects of IL-17 on colony-stimulating factor (CSF) release in human colonic subepithelial myofibroblasts (SEMFs). Methods. CSF release and mRNA expression were determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting, respectively. Nuclear factor (NF)-κB- and activating protein (AP-1)-DNA binding activities were evaluated by electrophoretic gel mobility shift assays (EMSAs). Results. Unstimulated cells secreted as mall amount of granulocyte G- and granulocyte/macrophage (GM)-CSF, and a considerable amount of M-CSF. IL-17 weakly enhanced G-CSF release, but did not affect GM- and M-CSF release. IL-17 selectively enhanced tumor necrosis factor (TNF)-α-induced G- and GM-CSF release. The combination of IL-17 plus TNF-α induced a marked increase in NF-κB- and AP-1-DNA binding activities. The adenovirus-mediated transfer of a stable form of IκBα and/or a dominant negative mutant of c-Jun markedly inhibited the IL-17 plus TNF-α-induced G- and GM-CSF mRNA expression. Furthermore, a stability study showed that IL-17 plus TNF-α markedly enhanced the stability of G- and GM-CSF mRNA. Conclusions. IL-17 augments TNF-α-induced G- and GM-CSF release via transcriptional and posttranscriptional mechanisms.

AB - Background. Interleukin (IL)-17 is a newly identified T-cell-specific cytokine. In this study, we investigated the effects of IL-17 on colony-stimulating factor (CSF) release in human colonic subepithelial myofibroblasts (SEMFs). Methods. CSF release and mRNA expression were determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting, respectively. Nuclear factor (NF)-κB- and activating protein (AP-1)-DNA binding activities were evaluated by electrophoretic gel mobility shift assays (EMSAs). Results. Unstimulated cells secreted as mall amount of granulocyte G- and granulocyte/macrophage (GM)-CSF, and a considerable amount of M-CSF. IL-17 weakly enhanced G-CSF release, but did not affect GM- and M-CSF release. IL-17 selectively enhanced tumor necrosis factor (TNF)-α-induced G- and GM-CSF release. The combination of IL-17 plus TNF-α induced a marked increase in NF-κB- and AP-1-DNA binding activities. The adenovirus-mediated transfer of a stable form of IκBα and/or a dominant negative mutant of c-Jun markedly inhibited the IL-17 plus TNF-α-induced G- and GM-CSF mRNA expression. Furthermore, a stability study showed that IL-17 plus TNF-α markedly enhanced the stability of G- and GM-CSF mRNA. Conclusions. IL-17 augments TNF-α-induced G- and GM-CSF release via transcriptional and posttranscriptional mechanisms.

KW - AP-1

KW - M-CSF

KW - NF-κB

KW - T cells

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