Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts.

Zhuobin Zhang, Akira Andoh, Hirofumi Yasui, Osamu Inatomi, Kazunori Hata, Tomoyuki Tsujikawa, Katsuyuki Kitoh, Atsushi Takayanagi, Nobuyoshi Shimizu, Yoshihide Fujiyama

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The recently identified cytokine interleukin-23 (IL-23) consists of p19 and p40 subunits. The major cellular source of IL-23 is dendritic cells and/or macrophages. We investigated the expression of IL-23 p19 mRNA in human colonic subepithelial myofibroblasts (SEMFs). p19 mRNA was not expressed in unstimulated SEMFs, but IL-1beta and TNF-alpha strongly induced p19 mRNA expression in these cells. The effects of IL-1beta were much stronger than those of TNF-alpha. These responses were observed in both a dose- and time-dependent manner. Furthermore, these cytokines acted synergistically when used in combination. A blockade of NF-kappaB activation by the overexpression of a stable form of IkappaBalpha completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1beta and TNF-alpha was mediated by the NF-kappaB activation pathway. In conclusion, this is the first report demonstrating that IL-23 p19 mRNA is inducible in colonic myofibroblasts by IL-1beta and TNF-alpha. The p19 expression in these cells might play a role in mucosal immune responses.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume15
Issue number1
Publication statusPublished - 2005 Jan

Fingerprint

Interleukin-23 Subunit p19
Myofibroblasts
Interleukin-1beta
Interleukin-23
Up-Regulation
Tumor Necrosis Factor-alpha
Gene Expression
Messenger RNA
NF-kappa B
Interleukin-12 Subunit p40
Cytokines
Mucosal Immunity
Dendritic Cells
Macrophages

ASJC Scopus subject areas

  • Genetics

Cite this

Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts. / Zhang, Zhuobin; Andoh, Akira; Yasui, Hirofumi; Inatomi, Osamu; Hata, Kazunori; Tsujikawa, Tomoyuki; Kitoh, Katsuyuki; Takayanagi, Atsushi; Shimizu, Nobuyoshi; Fujiyama, Yoshihide.

In: International Journal of Molecular Medicine, Vol. 15, No. 1, 01.2005, p. 79-83.

Research output: Contribution to journalArticle

Zhang, Z, Andoh, A, Yasui, H, Inatomi, O, Hata, K, Tsujikawa, T, Kitoh, K, Takayanagi, A, Shimizu, N & Fujiyama, Y 2005, 'Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts.', International Journal of Molecular Medicine, vol. 15, no. 1, pp. 79-83.
Zhang, Zhuobin ; Andoh, Akira ; Yasui, Hirofumi ; Inatomi, Osamu ; Hata, Kazunori ; Tsujikawa, Tomoyuki ; Kitoh, Katsuyuki ; Takayanagi, Atsushi ; Shimizu, Nobuyoshi ; Fujiyama, Yoshihide. / Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts. In: International Journal of Molecular Medicine. 2005 ; Vol. 15, No. 1. pp. 79-83.
@article{d5d5a4ac66934e99aa110d9d1c0309d8,
title = "Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts.",
abstract = "The recently identified cytokine interleukin-23 (IL-23) consists of p19 and p40 subunits. The major cellular source of IL-23 is dendritic cells and/or macrophages. We investigated the expression of IL-23 p19 mRNA in human colonic subepithelial myofibroblasts (SEMFs). p19 mRNA was not expressed in unstimulated SEMFs, but IL-1beta and TNF-alpha strongly induced p19 mRNA expression in these cells. The effects of IL-1beta were much stronger than those of TNF-alpha. These responses were observed in both a dose- and time-dependent manner. Furthermore, these cytokines acted synergistically when used in combination. A blockade of NF-kappaB activation by the overexpression of a stable form of IkappaBalpha completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1beta and TNF-alpha was mediated by the NF-kappaB activation pathway. In conclusion, this is the first report demonstrating that IL-23 p19 mRNA is inducible in colonic myofibroblasts by IL-1beta and TNF-alpha. The p19 expression in these cells might play a role in mucosal immune responses.",
author = "Zhuobin Zhang and Akira Andoh and Hirofumi Yasui and Osamu Inatomi and Kazunori Hata and Tomoyuki Tsujikawa and Katsuyuki Kitoh and Atsushi Takayanagi and Nobuyoshi Shimizu and Yoshihide Fujiyama",
year = "2005",
month = "1",
language = "English",
volume = "15",
pages = "79--83",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts.

AU - Zhang, Zhuobin

AU - Andoh, Akira

AU - Yasui, Hirofumi

AU - Inatomi, Osamu

AU - Hata, Kazunori

AU - Tsujikawa, Tomoyuki

AU - Kitoh, Katsuyuki

AU - Takayanagi, Atsushi

AU - Shimizu, Nobuyoshi

AU - Fujiyama, Yoshihide

PY - 2005/1

Y1 - 2005/1

N2 - The recently identified cytokine interleukin-23 (IL-23) consists of p19 and p40 subunits. The major cellular source of IL-23 is dendritic cells and/or macrophages. We investigated the expression of IL-23 p19 mRNA in human colonic subepithelial myofibroblasts (SEMFs). p19 mRNA was not expressed in unstimulated SEMFs, but IL-1beta and TNF-alpha strongly induced p19 mRNA expression in these cells. The effects of IL-1beta were much stronger than those of TNF-alpha. These responses were observed in both a dose- and time-dependent manner. Furthermore, these cytokines acted synergistically when used in combination. A blockade of NF-kappaB activation by the overexpression of a stable form of IkappaBalpha completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1beta and TNF-alpha was mediated by the NF-kappaB activation pathway. In conclusion, this is the first report demonstrating that IL-23 p19 mRNA is inducible in colonic myofibroblasts by IL-1beta and TNF-alpha. The p19 expression in these cells might play a role in mucosal immune responses.

AB - The recently identified cytokine interleukin-23 (IL-23) consists of p19 and p40 subunits. The major cellular source of IL-23 is dendritic cells and/or macrophages. We investigated the expression of IL-23 p19 mRNA in human colonic subepithelial myofibroblasts (SEMFs). p19 mRNA was not expressed in unstimulated SEMFs, but IL-1beta and TNF-alpha strongly induced p19 mRNA expression in these cells. The effects of IL-1beta were much stronger than those of TNF-alpha. These responses were observed in both a dose- and time-dependent manner. Furthermore, these cytokines acted synergistically when used in combination. A blockade of NF-kappaB activation by the overexpression of a stable form of IkappaBalpha completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1beta and TNF-alpha was mediated by the NF-kappaB activation pathway. In conclusion, this is the first report demonstrating that IL-23 p19 mRNA is inducible in colonic myofibroblasts by IL-1beta and TNF-alpha. The p19 expression in these cells might play a role in mucosal immune responses.

UR - http://www.scopus.com/inward/record.url?scp=21644446281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21644446281&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 79

EP - 83

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 1

ER -