Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts.

Zhuobin Zhang, Akira Andoh, Hirofumi Yasui, Osamu Inatomi, Kazunori Hata, Tomoyuki Tsujikawa, Katsuyuki Kitoh, Atsushi Takayanagi, Nobuyoshi Shimizu, Yoshihide Fujiyama

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25 Citations (Scopus)

Abstract

The recently identified cytokine interleukin-23 (IL-23) consists of p19 and p40 subunits. The major cellular source of IL-23 is dendritic cells and/or macrophages. We investigated the expression of IL-23 p19 mRNA in human colonic subepithelial myofibroblasts (SEMFs). p19 mRNA was not expressed in unstimulated SEMFs, but IL-1beta and TNF-alpha strongly induced p19 mRNA expression in these cells. The effects of IL-1beta were much stronger than those of TNF-alpha. These responses were observed in both a dose- and time-dependent manner. Furthermore, these cytokines acted synergistically when used in combination. A blockade of NF-kappaB activation by the overexpression of a stable form of IkappaBalpha completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1beta and TNF-alpha was mediated by the NF-kappaB activation pathway. In conclusion, this is the first report demonstrating that IL-23 p19 mRNA is inducible in colonic myofibroblasts by IL-1beta and TNF-alpha. The p19 expression in these cells might play a role in mucosal immune responses.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalInternational journal of molecular medicine
Volume15
Issue number1
Publication statusPublished - 2005 Jan

ASJC Scopus subject areas

  • Genetics

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    Zhang, Z., Andoh, A., Yasui, H., Inatomi, O., Hata, K., Tsujikawa, T., Kitoh, K., Takayanagi, A., Shimizu, N., & Fujiyama, Y. (2005). Interleukin-1beta and tumor necrosis factor-alpha upregulate interleukin-23 subunit p19 gene expression in human colonic subepithelial myofibroblasts. International journal of molecular medicine, 15(1), 79-83.