Interleukin-3 induces proliferation but not lymphokine activated killer activity from human and murine mononuclear cells.

D. M. Jablons, R. Donohue, Yutaka Kawakami, J. D. Young, M. T. Lotze

Research output: Contribution to journalArticle

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Abstract

Recombinant IL-3 (rIL-3) is a potent colony stimulating factor capable of stimulating early hematopoietic pluripotential progenitor cells and of supporting the differentiation of multiple cells. IL-3 has also been shown to have effects on mature, differentiated circulating cells including eosinophils and T cells. We evaluated the role of exogenous rIL-3 in the generation of cells with LAK activity from murine splenocytes and human bone marrow, spleen, unseparated PBMC and purified null cell preparations. rIL-3 was unable to generate lytic activity from any of these populations by itself and appeared to decrease LAK activity in bone marrow cultures containing high dose IL-2, (bone marrow derived cells (n = 3) with LAK activity for fresh tumor, mean lytic units(LU) 94.6 +/- 63.5 vs 32.8 +/- 44.8 for IL-2 and IL-2 plus IL-3 cultures, respectively p2 less than 0.05). Unlike previous reports testing murine cells, IL-3 priming and subsequent culture in IL-2 of human unseparated bone marrow cells or human or murine splenocytes, failed to generate long-term cultures with lytic activity. IL-3 did, however, induce a dose dependent stimulation of bone marrow and null cell preparations (mean null cell stimulation (3H Thymidine incorporation) with IL-3, 436 +/- 168 cpm vs 9802 +/- 9799 cpm, for 0 vs 10(3) units of IL-3, respectively n = 4, p2 less than 0.05). Furthermore, in bone marrow, unseparated PBMC and null cell cultures, the addition of rIL-3 generated characteristic large blastic appearing cells with prominent basophilic granules.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalEuropean Cytokine Network
Volume1
Issue number2
Publication statusPublished - 1990 May
Externally publishedYes

Fingerprint

Lymphokines
Interleukin-3
Human Activities
Null Lymphocytes
Bone
Interleukin-2
Bone Marrow Cells
Bone Marrow
Cells
Lymphokine-Activated Killer Cells
Colony-Stimulating Factors
T-cells
Hematopoietic Stem Cells
Cell culture
Eosinophils
Thymidine
Tumors
Cell Differentiation
Spleen
Cell Culture Techniques

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Interleukin-3 induces proliferation but not lymphokine activated killer activity from human and murine mononuclear cells. / Jablons, D. M.; Donohue, R.; Kawakami, Yutaka; Young, J. D.; Lotze, M. T.

In: European Cytokine Network, Vol. 1, No. 2, 05.1990, p. 91-98.

Research output: Contribution to journalArticle

Jablons, D. M. ; Donohue, R. ; Kawakami, Yutaka ; Young, J. D. ; Lotze, M. T. / Interleukin-3 induces proliferation but not lymphokine activated killer activity from human and murine mononuclear cells. In: European Cytokine Network. 1990 ; Vol. 1, No. 2. pp. 91-98.
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