Interleukin-4 and IgE in seasonal allergic conjunctivitis

Hiroshi Fujishima; Jun Shimazaki; Tsutomu Takeuchi; Ichiro Saito; Kazuo Tsubota

doi:10.1159/000310733

Interleukin-4 and IgE in seasonal allergic conjunctivitis

Hiroshi Fujishima, Jun Shimazaki, Tsutomu Takeuchi, Ichiro Saito, Kazuo Tsubota

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We measured interleukin-4 (IL-4) and IgE to study their presence in patients with seasonal allergic conjunctivitis. Twenty patients and 10 normal subjects were enrolled in this study, and their brush cytology samples and peripheral blood lymphocytes (PBL) were cultured for 3 or 24 h. IL-4 levels were measured using ELISA in the supernatants. Measurements of serum IgE were also performed. IL-4 was detected in 4 of 40 brush cytology samples and 10 of 40 PBL samples, but not from the controls. IL-4 positivity rate of patients was significantly higher than normal in 24-hour cultured PBL (p = 0.03). Patients’ serum IgE was also significantly higher (p = 0.006). IL-4 may contribute to the development of seasonal allergic conjunctivitis.

Original language	English
Pages (from-to)	325-328
Number of pages	4
Journal	Ophthalmologica
Volume	210
Issue number	6
DOIs	https://doi.org/10.1159/000310733
Publication status	Published - 1996 Jan 1
Externally published	Yes

Keywords

Allergic conjunctivitis
Brush cytology
Enzyme-linked immunosorbent assay
Interleukin-4
Peripheral blood lymphocytes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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abstract = "We measured interleukin-4 (IL-4) and IgE to study their presence in patients with seasonal allergic conjunctivitis. Twenty patients and 10 normal subjects were enrolled in this study, and their brush cytology samples and peripheral blood lymphocytes (PBL) were cultured for 3 or 24 h. IL-4 levels were measured using ELISA in the supernatants. Measurements of serum IgE were also performed. IL-4 was detected in 4 of 40 brush cytology samples and 10 of 40 PBL samples, but not from the controls. IL-4 positivity rate of patients was significantly higher than normal in 24-hour cultured PBL (p = 0.03). Patients{\textquoteright} serum IgE was also significantly higher (p = 0.006). IL-4 may contribute to the development of seasonal allergic conjunctivitis.",

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AU - Fujishima, Hiroshi

AU - Shimazaki, Jun

AU - Takeuchi, Tsutomu

AU - Saito, Ichiro

AU - Tsubota, Kazuo

PY - 1996/1/1

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N2 - We measured interleukin-4 (IL-4) and IgE to study their presence in patients with seasonal allergic conjunctivitis. Twenty patients and 10 normal subjects were enrolled in this study, and their brush cytology samples and peripheral blood lymphocytes (PBL) were cultured for 3 or 24 h. IL-4 levels were measured using ELISA in the supernatants. Measurements of serum IgE were also performed. IL-4 was detected in 4 of 40 brush cytology samples and 10 of 40 PBL samples, but not from the controls. IL-4 positivity rate of patients was significantly higher than normal in 24-hour cultured PBL (p = 0.03). Patients’ serum IgE was also significantly higher (p = 0.006). IL-4 may contribute to the development of seasonal allergic conjunctivitis.

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Interleukin-4 and IgE in seasonal allergic conjunctivitis

Abstract

Keywords

ASJC Scopus subject areas

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