Interleukin 4 downregulates cell growth and prostaglandin release of human mesangial cells

Yuichi Nakazato, Hirokazu Okada, Atsuhisa Sato, Yasushi Iwaita, Tomoko Hayashida, Matsuhiko Hayashi, Hiromichi Suzuki, Takao Saruta

Research output: Contribution to journalArticlepeer-review


Several clinical studies have proposed that a T cell derived cytokine IL-4 is operative in glomerulonephritis; however, its biological activities on renal cells have not been investigated. To elucidate its possible role in glomerulonephritis, we have examined whether IL-4 has effect on cell growth and prostaglandins synthesis using cultured mesangial cells (MC). IL-4 dose-dependently suppressed DNA synthesis and cell proliferation. IL-4 (10 ng/ml) alone did not affect prostaglandin E2 (PGE2) synthesis by mesangial cells, though, it inhibited IL-1α- and TNFα-stimulated PGE2 synthesis by 48% and 81%, respectively. Comparable inhibition was observed on the conversion of exogenous arachidonic acid to PGs by IL-1-stimulated cells, suggesting IL-4 down regulates PG endoperoxide synthase activity. These results demonstrated that IL-4 is antagonistic to inflammatory cytokines upon PG synthesis as well as anti-mitogenic with MC.

Original languageEnglish
Pages (from-to)486-493
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 1993 Dec 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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