Interleukin-7 contributes to human pro-B-cell development in a mouse stromal cell-dependent culture system

Tomoko Taguchi, Hisami Takenouchi, Yusuke Shiozawa, Jun Matsui, Noriko Kitamura, Yoshitaka Miyagawa, Yoko U. Katagiri, Takao Takahashi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

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Abstract

Objective: The role of interleukin (IL)-7 in human B lymphopoiesis is still controversial. We used an in vitro culture system to verify involvement of IL-7 in development of human pro-B cells from hematopoietic stem cells. Materials and Methods: Human CD34+ bone marrow cells were cultured for 4 weeks on MS-5 mouse stromal cells to induce pro-B cells. Expression of IL-7 receptor α or other B-cell differentiation marker genes on cultured human CD34+bone marrow cells was investigated by reverse transcription polymerase chain reaction (RT-PCR). Colony assay of human CD34+ bone marrow cells was also performed to determine the effect of IL-7 on colony-forming ability. Neutralizing antibody or reagent that eliminates the effect of IL-7 was added to the culture system, and the number of pro-B cells induced was estimated by flow cytometry. Results: RT-PCR analysis revealed mRNA expression of IL-7 receptor α as well as B-cell differentiation marker genes in not only CD19+ pro-B cells but also CD19- CD33- cells induced from CD34+ bone marrow cells after cultivation for 4 weeks on MS-5 cells. Addition of anti-mouse IL-7 antibody, anti-human IL-7 receptor α antibody, or JAK3 kinase inhibitor reduced the number of pro-B cells induced, demonstrating that elimination of IL-7 reduces pro-B-cell development. Addition of anti-mouse IL-7 antibody emphasized the colony-forming ability of burst-forming unit erythroid cells. Conclusions: IL-7 produced by MS-5 cells is required for human pro-B-cell development from CD34+bone marrow cells in our culture system, and IL-7 appears to play a certain role in early human B lymphopoiesis.

Original languageEnglish
Pages (from-to)1398-1407
Number of pages10
JournalExperimental Hematology
Volume35
Issue number9
DOIs
Publication statusPublished - 2007 Sep

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Interleukin-7
B-Lymphoid Precursor Cells
Stromal Cells
Cell Culture Techniques
Bone Marrow Cells
Interleukin-7 Receptors
Lymphopoiesis
Aptitude
Differentiation Antigens
Reverse Transcription
Cell Differentiation
B-Lymphocytes
Polymerase Chain Reaction
Erythroid Cells
Antibodies
Human Development
Hematopoietic Stem Cells
Neutralizing Antibodies
Genes
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Taguchi, T., Takenouchi, H., Shiozawa, Y., Matsui, J., Kitamura, N., Miyagawa, Y., ... Kiyokawa, N. (2007). Interleukin-7 contributes to human pro-B-cell development in a mouse stromal cell-dependent culture system. Experimental Hematology, 35(9), 1398-1407. https://doi.org/10.1016/j.exphem.2007.05.019

Interleukin-7 contributes to human pro-B-cell development in a mouse stromal cell-dependent culture system. / Taguchi, Tomoko; Takenouchi, Hisami; Shiozawa, Yusuke; Matsui, Jun; Kitamura, Noriko; Miyagawa, Yoshitaka; Katagiri, Yoko U.; Takahashi, Takao; Okita, Hajime; Fujimoto, Junichiro; Kiyokawa, Nobutaka.

In: Experimental Hematology, Vol. 35, No. 9, 09.2007, p. 1398-1407.

Research output: Contribution to journalArticle

Taguchi, T, Takenouchi, H, Shiozawa, Y, Matsui, J, Kitamura, N, Miyagawa, Y, Katagiri, YU, Takahashi, T, Okita, H, Fujimoto, J & Kiyokawa, N 2007, 'Interleukin-7 contributes to human pro-B-cell development in a mouse stromal cell-dependent culture system', Experimental Hematology, vol. 35, no. 9, pp. 1398-1407. https://doi.org/10.1016/j.exphem.2007.05.019
Taguchi, Tomoko ; Takenouchi, Hisami ; Shiozawa, Yusuke ; Matsui, Jun ; Kitamura, Noriko ; Miyagawa, Yoshitaka ; Katagiri, Yoko U. ; Takahashi, Takao ; Okita, Hajime ; Fujimoto, Junichiro ; Kiyokawa, Nobutaka. / Interleukin-7 contributes to human pro-B-cell development in a mouse stromal cell-dependent culture system. In: Experimental Hematology. 2007 ; Vol. 35, No. 9. pp. 1398-1407.
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AU - Kitamura, Noriko

AU - Miyagawa, Yoshitaka

AU - Katagiri, Yoko U.

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