Interleukin-8 and monocyte chemotactic protein-1 production by a human glioblastoma cell line, T98G in coculture with monocytes: Involvement of monocyte-derived interleukin-1-alpha

Tadashi Kasahara, Taiko Oda, Kiyohiko Hatake, Mariko Akiyama, Naofumi Mukaida, Kouji Matsushima

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have previously demonstrated that a human glioblastoma cell line, T98G cells, produced high levels of interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) when stimulated with IL-1 or tumor necrosis factor-α (TNF-α). In this study, we found that T98G cells are capable of producing large amounts of IL-8 and MCP-1 when cocultured with human peripheral blood monocytes or a monocytic cell line, U937 cells. Since it is possible that both glioblastoma cells and monocytes are capable of producing chemokines, we determined which type of cells actually produced IL-8 and MCP-1, by the fixation of one or the other cell type with 3% paraformaldehyde (PA). This procedure revealed that T98G cells were the main source and that PA-treated monocytes effectively stimulated IL-8 and MCP-1 production by T98G cells. Both IL-8 and MCP-1 gene expression and protein production by T98G cells were confirmed by northern blot as well as immunohistochemical staining methods. To analyze the molecules on human monocytes responsible for inducing IL-8 and MCP-1 by T98G cells, several antibodies (Abs) as well as IL-1 receptor antagonist (IL-1Ra) were tested. Anti-IL-1α Ab and IL-1Ra almost completely abolished the IL-8/MCP-1-inducing capacity of the PA-fixed monocytes, while no inhibition was obtained with anti-IL-1β, anti-TNF-α or Abs against CD11b/18, L-selectin or ICAM-1, indicating that membrane-associated IL-1α is involved in the IL-8/MCP-1 induction, while secreted IL-1α plays a major role in this cell-to-cell, i.e., juxtacrine interaction in unfixed conditions.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalEuropean Cytokine Network
Volume9
Issue number1
Publication statusPublished - 1998

Fingerprint

Interleukin-1alpha
Chemokine CCL2
Glioblastoma
Coculture Techniques
Interleukin-8
Monocytes
Cells
Cell Line
Interleukin-1
Interleukin-1 Receptors
Tumor Necrosis Factor-alpha
L-Selectin
Antibodies
Intercellular Adhesion Molecule-1
Chemokines
Gene expression
U937 Cells
Blood
Northern Blotting
Membranes

Keywords

  • Glioblastoma
  • IL-1 receptor antagonist
  • IL-1α
  • IL-8
  • MCP-1

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Interleukin-8 and monocyte chemotactic protein-1 production by a human glioblastoma cell line, T98G in coculture with monocytes : Involvement of monocyte-derived interleukin-1-alpha. / Kasahara, Tadashi; Oda, Taiko; Hatake, Kiyohiko; Akiyama, Mariko; Mukaida, Naofumi; Matsushima, Kouji.

In: European Cytokine Network, Vol. 9, No. 1, 1998, p. 47-55.

Research output: Contribution to journalArticle

Kasahara, Tadashi ; Oda, Taiko ; Hatake, Kiyohiko ; Akiyama, Mariko ; Mukaida, Naofumi ; Matsushima, Kouji. / Interleukin-8 and monocyte chemotactic protein-1 production by a human glioblastoma cell line, T98G in coculture with monocytes : Involvement of monocyte-derived interleukin-1-alpha. In: European Cytokine Network. 1998 ; Vol. 9, No. 1. pp. 47-55.
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