Intermittent hypoxia induces disturbances in craniofacial growth and defects in craniofacial morphology

Shuji Oishi, Yasuhiro Shimizu, Jun Hosomichi, Yoichiro Kuma, Hisashi Nagai, Hideyuki Maeda, Risa Usumi-Fujita, Sawa Kaneko, Chisa Shitano, Jun Ichi Suzuki, Ken Ichi Yoshida, Takashi Ono

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objectives To investigate intermittent hypoxia (IH) induced changes in craniofacial morphology and bone mineral density (BMD) in the mandible of growing rats. Design Seven-week-old male Sprague-Dawley rats were exposed to IH for 4 days or 3 weeks. Sham-operated rats simultaneously breathed room air. Lateral and transverse cephalometric radiographs of the craniofacial region were obtained, and the linear distances between cephalometric landmarks were statistically analyzed. BMD and bone microstructure of the mandible were evaluated using micro-computed tomography (micro-CT). Results Cephalometric analyses demonstrated that exposure to IH only in the two groups for 3 weeks decreased the size of the mandibular and viscerocranial bones, but not that of the neurocranial bones, in early adolescent rats. These findings are consistent with upper airway narrowing and obstructive sleep apnea (OSA). Micro-CT showed that IH increased the BMD in the cancellous bone of the mandibular condyle and the inter-radicular alveolar bone in the mandibular first molar (M1) region. Conclusions This study is the first to identify growth retardation of the craniofacial bones in an animal model of sleep apnea. Notably, 3 weeks of IH can induce multiple changes in the bones around the upper airway in pubertal rats, which can enhance upper airway narrowing and the development of OSA. The reproducibility of these results supports the validity and usefulness of this model. These findings also emphasize the critical importance of morphometric evaluation of patients with OSA.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalArchives of Oral Biology
Volume61
DOIs
Publication statusPublished - 2016 Jan 1
Externally publishedYes

Fingerprint

Cephalometry
Bone and Bones
Obstructive Sleep Apnea
Growth
Bone Density
Mandible
Mandibular Condyle
Sleep Apnea Syndromes
Reproducibility of Results
Sprague Dawley Rats
Hypoxia
Animal Models
Air
Tomography

Keywords

  • Bone mineral density
  • Craniofacial growth
  • Growth retardation
  • Intermittent hypoxia
  • Micro-CT analysis
  • Obstructive sleep apnea

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Intermittent hypoxia induces disturbances in craniofacial growth and defects in craniofacial morphology. / Oishi, Shuji; Shimizu, Yasuhiro; Hosomichi, Jun; Kuma, Yoichiro; Nagai, Hisashi; Maeda, Hideyuki; Usumi-Fujita, Risa; Kaneko, Sawa; Shitano, Chisa; Suzuki, Jun Ichi; Yoshida, Ken Ichi; Ono, Takashi.

In: Archives of Oral Biology, Vol. 61, 01.01.2016, p. 115-124.

Research output: Contribution to journalArticle

Oishi, S, Shimizu, Y, Hosomichi, J, Kuma, Y, Nagai, H, Maeda, H, Usumi-Fujita, R, Kaneko, S, Shitano, C, Suzuki, JI, Yoshida, KI & Ono, T 2016, 'Intermittent hypoxia induces disturbances in craniofacial growth and defects in craniofacial morphology', Archives of Oral Biology, vol. 61, pp. 115-124. https://doi.org/10.1016/j.archoralbio.2015.10.017
Oishi, Shuji ; Shimizu, Yasuhiro ; Hosomichi, Jun ; Kuma, Yoichiro ; Nagai, Hisashi ; Maeda, Hideyuki ; Usumi-Fujita, Risa ; Kaneko, Sawa ; Shitano, Chisa ; Suzuki, Jun Ichi ; Yoshida, Ken Ichi ; Ono, Takashi. / Intermittent hypoxia induces disturbances in craniofacial growth and defects in craniofacial morphology. In: Archives of Oral Biology. 2016 ; Vol. 61. pp. 115-124.
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abstract = "Objectives To investigate intermittent hypoxia (IH) induced changes in craniofacial morphology and bone mineral density (BMD) in the mandible of growing rats. Design Seven-week-old male Sprague-Dawley rats were exposed to IH for 4 days or 3 weeks. Sham-operated rats simultaneously breathed room air. Lateral and transverse cephalometric radiographs of the craniofacial region were obtained, and the linear distances between cephalometric landmarks were statistically analyzed. BMD and bone microstructure of the mandible were evaluated using micro-computed tomography (micro-CT). Results Cephalometric analyses demonstrated that exposure to IH only in the two groups for 3 weeks decreased the size of the mandibular and viscerocranial bones, but not that of the neurocranial bones, in early adolescent rats. These findings are consistent with upper airway narrowing and obstructive sleep apnea (OSA). Micro-CT showed that IH increased the BMD in the cancellous bone of the mandibular condyle and the inter-radicular alveolar bone in the mandibular first molar (M1) region. Conclusions This study is the first to identify growth retardation of the craniofacial bones in an animal model of sleep apnea. Notably, 3 weeks of IH can induce multiple changes in the bones around the upper airway in pubertal rats, which can enhance upper airway narrowing and the development of OSA. The reproducibility of these results supports the validity and usefulness of this model. These findings also emphasize the critical importance of morphometric evaluation of patients with OSA.",
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N2 - Objectives To investigate intermittent hypoxia (IH) induced changes in craniofacial morphology and bone mineral density (BMD) in the mandible of growing rats. Design Seven-week-old male Sprague-Dawley rats were exposed to IH for 4 days or 3 weeks. Sham-operated rats simultaneously breathed room air. Lateral and transverse cephalometric radiographs of the craniofacial region were obtained, and the linear distances between cephalometric landmarks were statistically analyzed. BMD and bone microstructure of the mandible were evaluated using micro-computed tomography (micro-CT). Results Cephalometric analyses demonstrated that exposure to IH only in the two groups for 3 weeks decreased the size of the mandibular and viscerocranial bones, but not that of the neurocranial bones, in early adolescent rats. These findings are consistent with upper airway narrowing and obstructive sleep apnea (OSA). Micro-CT showed that IH increased the BMD in the cancellous bone of the mandibular condyle and the inter-radicular alveolar bone in the mandibular first molar (M1) region. Conclusions This study is the first to identify growth retardation of the craniofacial bones in an animal model of sleep apnea. Notably, 3 weeks of IH can induce multiple changes in the bones around the upper airway in pubertal rats, which can enhance upper airway narrowing and the development of OSA. The reproducibility of these results supports the validity and usefulness of this model. These findings also emphasize the critical importance of morphometric evaluation of patients with OSA.

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