Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor

S. Miura, H. Tashiro, I. Kurose, M. Suematsu, H. Serizawa, E. Sekizuka, H. Nagata, M. Yoshioka, M. Tsuchiya

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The major objective of this study was to estimate how microvascular changes occur in the intestinal segment in relation to fat absorption and also to assess the role played by cholecystokinin (CCK) in fat-induced intestinal hyperemia. A 12-cm loop of rat middle small intestine was exposed to a 120-min infusion of oleic acid micelle solution containing [14C]oleic acid. Time-course changes of vascular diameter and red blood cell (RBC) velocity of submucosal arterioles, venules, and periglandular and muscular capillaries were determined simultaneously in their different order of branching by using intravital microscopy equipped with a high-speed video camera system. Lymphatic transport of oleic acid was also monitored by collecting lymph from intestinal lymphatics. The instillation of micelle produced significant dilatation of submucosal arterioles and venules and significant increase in RBC velocity in submucosal microvessels and periglandular capillaries at 15 to 30 min after the fat instillation. This corresponds well to the appearance of [14C]oleic acid in intestinal lymph. This microvascular dilatation and RBC velocity increase continued throughout the exposure to fat and started to attenuate 30 min after restoration to saline infusion. There was also heterogeneity in microvascular responses among different orders of branches to fat administration, and RBC velocity increase was not observed in muscular capillaries. Local intra-arterial infusion of CCK inhibitor, loxiglumide (CR 1505, 10 mg · kg-1 · h-1), significantly attenuated both fat-induced microvascular dilatation and RBC velocity increase but not the fat absorptive function. Our results indicate that CCK plays a significant role in intestinal hyperemia induced by fat absorption. However, CCK-mediated intestinal microvascular change was not a prerequisite for fat absorption.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume262
Issue number3 25-3
Publication statusPublished - 1992

Fingerprint

Cholecystokinin
Fats
Oleic Acid
Erythrocytes
Dilatation
Venules
Hyperemia
Arterioles
Micelles
Lymph
Intra Arterial Infusions
Microvessels
Small Intestine
Blood Vessels

Keywords

  • high-speed video camera system
  • loxiglumide
  • lymphatic fatty acid transport

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Miura, S., Tashiro, H., Kurose, I., Suematsu, M., Serizawa, H., Sekizuka, E., ... Tsuchiya, M. (1992). Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor. American Journal of Physiology - Gastrointestinal and Liver Physiology, 262(3 25-3).

Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor. / Miura, S.; Tashiro, H.; Kurose, I.; Suematsu, M.; Serizawa, H.; Sekizuka, E.; Nagata, H.; Yoshioka, M.; Tsuchiya, M.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 262, No. 3 25-3, 1992.

Research output: Contribution to journalArticle

Miura, S, Tashiro, H, Kurose, I, Suematsu, M, Serizawa, H, Sekizuka, E, Nagata, H, Yoshioka, M & Tsuchiya, M 1992, 'Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 262, no. 3 25-3.
Miura, S. ; Tashiro, H. ; Kurose, I. ; Suematsu, M. ; Serizawa, H. ; Sekizuka, E. ; Nagata, H. ; Yoshioka, M. ; Tsuchiya, M. / Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1992 ; Vol. 262, No. 3 25-3.
@article{da6bb01bdfdb4686adeb2b0dc356a8b1,
title = "Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor",
abstract = "The major objective of this study was to estimate how microvascular changes occur in the intestinal segment in relation to fat absorption and also to assess the role played by cholecystokinin (CCK) in fat-induced intestinal hyperemia. A 12-cm loop of rat middle small intestine was exposed to a 120-min infusion of oleic acid micelle solution containing [14C]oleic acid. Time-course changes of vascular diameter and red blood cell (RBC) velocity of submucosal arterioles, venules, and periglandular and muscular capillaries were determined simultaneously in their different order of branching by using intravital microscopy equipped with a high-speed video camera system. Lymphatic transport of oleic acid was also monitored by collecting lymph from intestinal lymphatics. The instillation of micelle produced significant dilatation of submucosal arterioles and venules and significant increase in RBC velocity in submucosal microvessels and periglandular capillaries at 15 to 30 min after the fat instillation. This corresponds well to the appearance of [14C]oleic acid in intestinal lymph. This microvascular dilatation and RBC velocity increase continued throughout the exposure to fat and started to attenuate 30 min after restoration to saline infusion. There was also heterogeneity in microvascular responses among different orders of branches to fat administration, and RBC velocity increase was not observed in muscular capillaries. Local intra-arterial infusion of CCK inhibitor, loxiglumide (CR 1505, 10 mg · kg-1 · h-1), significantly attenuated both fat-induced microvascular dilatation and RBC velocity increase but not the fat absorptive function. Our results indicate that CCK plays a significant role in intestinal hyperemia induced by fat absorption. However, CCK-mediated intestinal microvascular change was not a prerequisite for fat absorption.",
keywords = "high-speed video camera system, loxiglumide, lymphatic fatty acid transport",
author = "S. Miura and H. Tashiro and I. Kurose and M. Suematsu and H. Serizawa and E. Sekizuka and H. Nagata and M. Yoshioka and M. Tsuchiya",
year = "1992",
language = "English",
volume = "262",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 25-3",

}

TY - JOUR

T1 - Intestinal microcirculatory changes during fat absorption and the effect of cholecystokinin inhibitor

AU - Miura, S.

AU - Tashiro, H.

AU - Kurose, I.

AU - Suematsu, M.

AU - Serizawa, H.

AU - Sekizuka, E.

AU - Nagata, H.

AU - Yoshioka, M.

AU - Tsuchiya, M.

PY - 1992

Y1 - 1992

N2 - The major objective of this study was to estimate how microvascular changes occur in the intestinal segment in relation to fat absorption and also to assess the role played by cholecystokinin (CCK) in fat-induced intestinal hyperemia. A 12-cm loop of rat middle small intestine was exposed to a 120-min infusion of oleic acid micelle solution containing [14C]oleic acid. Time-course changes of vascular diameter and red blood cell (RBC) velocity of submucosal arterioles, venules, and periglandular and muscular capillaries were determined simultaneously in their different order of branching by using intravital microscopy equipped with a high-speed video camera system. Lymphatic transport of oleic acid was also monitored by collecting lymph from intestinal lymphatics. The instillation of micelle produced significant dilatation of submucosal arterioles and venules and significant increase in RBC velocity in submucosal microvessels and periglandular capillaries at 15 to 30 min after the fat instillation. This corresponds well to the appearance of [14C]oleic acid in intestinal lymph. This microvascular dilatation and RBC velocity increase continued throughout the exposure to fat and started to attenuate 30 min after restoration to saline infusion. There was also heterogeneity in microvascular responses among different orders of branches to fat administration, and RBC velocity increase was not observed in muscular capillaries. Local intra-arterial infusion of CCK inhibitor, loxiglumide (CR 1505, 10 mg · kg-1 · h-1), significantly attenuated both fat-induced microvascular dilatation and RBC velocity increase but not the fat absorptive function. Our results indicate that CCK plays a significant role in intestinal hyperemia induced by fat absorption. However, CCK-mediated intestinal microvascular change was not a prerequisite for fat absorption.

AB - The major objective of this study was to estimate how microvascular changes occur in the intestinal segment in relation to fat absorption and also to assess the role played by cholecystokinin (CCK) in fat-induced intestinal hyperemia. A 12-cm loop of rat middle small intestine was exposed to a 120-min infusion of oleic acid micelle solution containing [14C]oleic acid. Time-course changes of vascular diameter and red blood cell (RBC) velocity of submucosal arterioles, venules, and periglandular and muscular capillaries were determined simultaneously in their different order of branching by using intravital microscopy equipped with a high-speed video camera system. Lymphatic transport of oleic acid was also monitored by collecting lymph from intestinal lymphatics. The instillation of micelle produced significant dilatation of submucosal arterioles and venules and significant increase in RBC velocity in submucosal microvessels and periglandular capillaries at 15 to 30 min after the fat instillation. This corresponds well to the appearance of [14C]oleic acid in intestinal lymph. This microvascular dilatation and RBC velocity increase continued throughout the exposure to fat and started to attenuate 30 min after restoration to saline infusion. There was also heterogeneity in microvascular responses among different orders of branches to fat administration, and RBC velocity increase was not observed in muscular capillaries. Local intra-arterial infusion of CCK inhibitor, loxiglumide (CR 1505, 10 mg · kg-1 · h-1), significantly attenuated both fat-induced microvascular dilatation and RBC velocity increase but not the fat absorptive function. Our results indicate that CCK plays a significant role in intestinal hyperemia induced by fat absorption. However, CCK-mediated intestinal microvascular change was not a prerequisite for fat absorption.

KW - high-speed video camera system

KW - loxiglumide

KW - lymphatic fatty acid transport

UR - http://www.scopus.com/inward/record.url?scp=0026552631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026552631&partnerID=8YFLogxK

M3 - Article

C2 - 1550231

AN - SCOPUS:0026552631

VL - 262

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 3 25-3

ER -