Intra-tympanic delivery of short interfering RNA into the adult mouse cochlea

Naoki Oishi, Fu Quan Chen, Hong Wei Zheng, Su Hua Sha

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Trans-tympanic injection into the middle ear has long been the standard for local delivery of compounds in experimental studies. Here we demonstrate the advantages of the novel method of intra-tympanic injection through the otic bone for the delivery of compounds or siRNA into the adult mouse cochlea. First, a fluorescently-conjugated scrambled siRNA probe was applied via intra-tympanic injection into the middle ear cavity and was detected in sensory hair cells and nerve fibers as early as 6 h after the injection. The fluorescent probe was also detected in other cells of the organ of Corti, the lateral wall, and in spiral ganglion cells 48 h after the injection. Furthermore, intra-tympanic delivery of Nox3 siRNA successfully reduced immunofluorescence associated with Nox3 in outer hair cells 72 h after injection by 20%. Drug or siRNA delivery via intra-tympanic injection does not compromise the tympanic membrane or interfere with noise-induced hearing loss, while trans-tympanic injections significantly altered the cochlear response to noise exposure. In summary, intra-tympanic injection through the otic bone into the middle ear cavity provides a promising approach for delivery of compounds or siRNA to cochlear hair cells of adult mice, relevant for the study of mechanisms underlying inner ear insults and, specifically, noise-induced hearing loss.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalHearing Research
Volume296
DOIs
Publication statusPublished - 2013 Feb
Externally publishedYes

Fingerprint

Cochlea
Small Interfering RNA
Injections
Middle Ear
Noise-Induced Hearing Loss
Outer Auditory Hair Cells
Ear
Auditory Hair Cells
Spiral Ganglion
Bone and Bones
Organ of Corti
Tympanic Membrane
Inner Ear
Fluorescent Dyes
Nerve Fibers
Fluorescent Antibody Technique
Noise

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Intra-tympanic delivery of short interfering RNA into the adult mouse cochlea. / Oishi, Naoki; Chen, Fu Quan; Zheng, Hong Wei; Sha, Su Hua.

In: Hearing Research, Vol. 296, 02.2013, p. 36-41.

Research output: Contribution to journalArticle

Oishi, Naoki ; Chen, Fu Quan ; Zheng, Hong Wei ; Sha, Su Hua. / Intra-tympanic delivery of short interfering RNA into the adult mouse cochlea. In: Hearing Research. 2013 ; Vol. 296. pp. 36-41.
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