Intractable ulcer caused by Mycobacterium shinshuense

Successful identification of mycobacterium strain by 16S ribosomal RNA 3′-end sequencing

Takeru Funakoshi, Y. Kazumi, R. Okada, K. Nishimoto, Masataka Saito, Masayuki Amagai, H. Shimura, M. Ohyama

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5 Citations (Scopus)

Abstract

An extremely rare case of intractable ulcer caused by Mycobacterium shinshuense is described. A 59-year-old Japanese woman developed an ulcerated subcutaneous induration on the upper arm. Ziehl-Neelsen staining revealed positive bacilli. Tissue culture isolated Mycobacterium species, but standard identification techniques (including molecular biological approaches such as DNA-DNA hybridization) could not distinguish the precise causative pathogen, although it was narrowed down to three possibilities: Mycobacterium marinum, Mycobacterium ulcerans and M. shinshuense. Finally, a novel 16S rRNA sequencing method enabled the diagnosis of M. shinshuense infection. The epidemiology of the cutaneous infection caused by this mycobacterium has yet to be elucidated, but a review of reported cases indicated that ulcers having some resemblance to those caused by M. ulcerans infection were found in nonendemic areas and that M. shinshuense could be considered as the cause. The approach introduced in this report could provide a powerful tool for the identification of this organism.

Original languageEnglish
JournalClinical and Experimental Dermatology
Volume34
Issue number8
DOIs
Publication statusPublished - 2009 Dec

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16S Ribosomal RNA
Mycobacterium
Ulcer
Mycobacterium ulcerans
Buruli Ulcer
Mycobacterium marinum
Mycobacterium Infections
DNA
Bacillus
Epidemiology
Arm
Staining and Labeling
Skin
Infection

ASJC Scopus subject areas

  • Dermatology

Cite this

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title = "Intractable ulcer caused by Mycobacterium shinshuense: Successful identification of mycobacterium strain by 16S ribosomal RNA 3′-end sequencing",
abstract = "An extremely rare case of intractable ulcer caused by Mycobacterium shinshuense is described. A 59-year-old Japanese woman developed an ulcerated subcutaneous induration on the upper arm. Ziehl-Neelsen staining revealed positive bacilli. Tissue culture isolated Mycobacterium species, but standard identification techniques (including molecular biological approaches such as DNA-DNA hybridization) could not distinguish the precise causative pathogen, although it was narrowed down to three possibilities: Mycobacterium marinum, Mycobacterium ulcerans and M. shinshuense. Finally, a novel 16S rRNA sequencing method enabled the diagnosis of M. shinshuense infection. The epidemiology of the cutaneous infection caused by this mycobacterium has yet to be elucidated, but a review of reported cases indicated that ulcers having some resemblance to those caused by M. ulcerans infection were found in nonendemic areas and that M. shinshuense could be considered as the cause. The approach introduced in this report could provide a powerful tool for the identification of this organism.",
author = "Takeru Funakoshi and Y. Kazumi and R. Okada and K. Nishimoto and Masataka Saito and Masayuki Amagai and H. Shimura and M. Ohyama",
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T2 - Successful identification of mycobacterium strain by 16S ribosomal RNA 3′-end sequencing

AU - Funakoshi, Takeru

AU - Kazumi, Y.

AU - Okada, R.

AU - Nishimoto, K.

AU - Saito, Masataka

AU - Amagai, Masayuki

AU - Shimura, H.

AU - Ohyama, M.

PY - 2009/12

Y1 - 2009/12

N2 - An extremely rare case of intractable ulcer caused by Mycobacterium shinshuense is described. A 59-year-old Japanese woman developed an ulcerated subcutaneous induration on the upper arm. Ziehl-Neelsen staining revealed positive bacilli. Tissue culture isolated Mycobacterium species, but standard identification techniques (including molecular biological approaches such as DNA-DNA hybridization) could not distinguish the precise causative pathogen, although it was narrowed down to three possibilities: Mycobacterium marinum, Mycobacterium ulcerans and M. shinshuense. Finally, a novel 16S rRNA sequencing method enabled the diagnosis of M. shinshuense infection. The epidemiology of the cutaneous infection caused by this mycobacterium has yet to be elucidated, but a review of reported cases indicated that ulcers having some resemblance to those caused by M. ulcerans infection were found in nonendemic areas and that M. shinshuense could be considered as the cause. The approach introduced in this report could provide a powerful tool for the identification of this organism.

AB - An extremely rare case of intractable ulcer caused by Mycobacterium shinshuense is described. A 59-year-old Japanese woman developed an ulcerated subcutaneous induration on the upper arm. Ziehl-Neelsen staining revealed positive bacilli. Tissue culture isolated Mycobacterium species, but standard identification techniques (including molecular biological approaches such as DNA-DNA hybridization) could not distinguish the precise causative pathogen, although it was narrowed down to three possibilities: Mycobacterium marinum, Mycobacterium ulcerans and M. shinshuense. Finally, a novel 16S rRNA sequencing method enabled the diagnosis of M. shinshuense infection. The epidemiology of the cutaneous infection caused by this mycobacterium has yet to be elucidated, but a review of reported cases indicated that ulcers having some resemblance to those caused by M. ulcerans infection were found in nonendemic areas and that M. shinshuense could be considered as the cause. The approach introduced in this report could provide a powerful tool for the identification of this organism.

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