Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs

Mitsuhiro Yanai, Erito Mochiki, Atsushi Ogawa, Hiroki Morita, Yoshitaka Toyomasu, Kyoichi Ogata, Yuichi Tabe, Hiroyuki Ando, Tetsuro Ohno, Takayuki Asao, Tohru Aomori, Yukiyoshi Fujita, Hiroyuki Kuwano

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Traditional Japanese medicine, known as Kampo medicine, consists of mixtures of several medicinal herbs widely used to treat upper gastrointestinal disorders in Japan. Rikkunshito, one of these medicines, has not been evaluated with respect to its influence on gastrointestinal motor activity. We investigated the effect of rikkunshito on upper gastrointestinal motility and plasma ghrelin concentrations in conscious dogs. Methods: Contractile response to intragastric administration of rikkunshito was studied via surgically implanted force transducers. A powdered extract of rikkunshito (1.3, 2.7, and 4.0 g) dissolved in water was administered into the stomachs of normal and vagotomized dogs before feeding and gastric emptying was evaluated. Several inhibitors of gastrointestinal motility (atropine, hexamethonium, and ondansetron) were injected intravenously before intragastric administration of rikkunshito. Plasma acylated ghrelin levels after intragastric administration of rikkunshito were measured. Results: In a fasting state, intragastric administration of rikkunshito induced phasic contractions in the duodenum and jejunum in normal dogs. Rikkunshito-induced contractions were inhibited by atropine, hexamethonium and ondansetron. In vagotomized dogs, rikkunshito induced phasic contractions, similar to normal dogs. Gastric emptying was accelerated by intragastric administration of rikkunshito in a dose-dependent manner. The plasma acylated ghrelin level 150 min after intragastric administration of 4.0 g of rikkunshito was significantly higher than the control value. Conclusions: Intragastric administration of rikkunshito stimulates gastrointestinal contractions in the interdigestive state through cholinergic neurons and 5-HT type 3 receptors. Moreover, rikkunshito increases plasma acylated ghrelin levels. Rikkunshito may alleviate gastrointestinal disorders through its prokinetic effects.

Original languageEnglish
Pages (from-to)611-619
Number of pages9
JournalJournal of Gastroenterology
Volume48
Issue number5
DOIs
Publication statusPublished - 2013 May
Externally publishedYes

Fingerprint

Gastrointestinal Motility
Gastric Emptying
Dogs
Ghrelin
Ondansetron
Hexamethonium
liu-jun-zi-tang
Atropine
Kampo Medicine
Cholinergic Neurons
Serotonin Receptors
Traditional Medicine
Jejunum
Medicinal Plants
Transducers
Duodenum
Fasting

Keywords

  • Gastric emptying
  • Gastrointestinal motility
  • Ghrelin
  • Rikkunshito

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs. / Yanai, Mitsuhiro; Mochiki, Erito; Ogawa, Atsushi; Morita, Hiroki; Toyomasu, Yoshitaka; Ogata, Kyoichi; Tabe, Yuichi; Ando, Hiroyuki; Ohno, Tetsuro; Asao, Takayuki; Aomori, Tohru; Fujita, Yukiyoshi; Kuwano, Hiroyuki.

In: Journal of Gastroenterology, Vol. 48, No. 5, 05.2013, p. 611-619.

Research output: Contribution to journalArticle

Yanai, M, Mochiki, E, Ogawa, A, Morita, H, Toyomasu, Y, Ogata, K, Tabe, Y, Ando, H, Ohno, T, Asao, T, Aomori, T, Fujita, Y & Kuwano, H 2013, 'Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs', Journal of Gastroenterology, vol. 48, no. 5, pp. 611-619. https://doi.org/10.1007/s00535-012-0687-8
Yanai, Mitsuhiro ; Mochiki, Erito ; Ogawa, Atsushi ; Morita, Hiroki ; Toyomasu, Yoshitaka ; Ogata, Kyoichi ; Tabe, Yuichi ; Ando, Hiroyuki ; Ohno, Tetsuro ; Asao, Takayuki ; Aomori, Tohru ; Fujita, Yukiyoshi ; Kuwano, Hiroyuki. / Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs. In: Journal of Gastroenterology. 2013 ; Vol. 48, No. 5. pp. 611-619.
@article{cfc03e0d6dc9432eadd0183d72d90a30,
title = "Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs",
abstract = "Background: Traditional Japanese medicine, known as Kampo medicine, consists of mixtures of several medicinal herbs widely used to treat upper gastrointestinal disorders in Japan. Rikkunshito, one of these medicines, has not been evaluated with respect to its influence on gastrointestinal motor activity. We investigated the effect of rikkunshito on upper gastrointestinal motility and plasma ghrelin concentrations in conscious dogs. Methods: Contractile response to intragastric administration of rikkunshito was studied via surgically implanted force transducers. A powdered extract of rikkunshito (1.3, 2.7, and 4.0 g) dissolved in water was administered into the stomachs of normal and vagotomized dogs before feeding and gastric emptying was evaluated. Several inhibitors of gastrointestinal motility (atropine, hexamethonium, and ondansetron) were injected intravenously before intragastric administration of rikkunshito. Plasma acylated ghrelin levels after intragastric administration of rikkunshito were measured. Results: In a fasting state, intragastric administration of rikkunshito induced phasic contractions in the duodenum and jejunum in normal dogs. Rikkunshito-induced contractions were inhibited by atropine, hexamethonium and ondansetron. In vagotomized dogs, rikkunshito induced phasic contractions, similar to normal dogs. Gastric emptying was accelerated by intragastric administration of rikkunshito in a dose-dependent manner. The plasma acylated ghrelin level 150 min after intragastric administration of 4.0 g of rikkunshito was significantly higher than the control value. Conclusions: Intragastric administration of rikkunshito stimulates gastrointestinal contractions in the interdigestive state through cholinergic neurons and 5-HT type 3 receptors. Moreover, rikkunshito increases plasma acylated ghrelin levels. Rikkunshito may alleviate gastrointestinal disorders through its prokinetic effects.",
keywords = "Gastric emptying, Gastrointestinal motility, Ghrelin, Rikkunshito",
author = "Mitsuhiro Yanai and Erito Mochiki and Atsushi Ogawa and Hiroki Morita and Yoshitaka Toyomasu and Kyoichi Ogata and Yuichi Tabe and Hiroyuki Ando and Tetsuro Ohno and Takayuki Asao and Tohru Aomori and Yukiyoshi Fujita and Hiroyuki Kuwano",
year = "2013",
month = "5",
doi = "10.1007/s00535-012-0687-8",
language = "English",
volume = "48",
pages = "611--619",
journal = "Journal of Gastroenterology",
issn = "0944-1174",
publisher = "Springer Japan",
number = "5",

}

TY - JOUR

T1 - Intragastric administration of rikkunshito stimulates upper gastrointestinal motility and gastric emptying in conscious dogs

AU - Yanai, Mitsuhiro

AU - Mochiki, Erito

AU - Ogawa, Atsushi

AU - Morita, Hiroki

AU - Toyomasu, Yoshitaka

AU - Ogata, Kyoichi

AU - Tabe, Yuichi

AU - Ando, Hiroyuki

AU - Ohno, Tetsuro

AU - Asao, Takayuki

AU - Aomori, Tohru

AU - Fujita, Yukiyoshi

AU - Kuwano, Hiroyuki

PY - 2013/5

Y1 - 2013/5

N2 - Background: Traditional Japanese medicine, known as Kampo medicine, consists of mixtures of several medicinal herbs widely used to treat upper gastrointestinal disorders in Japan. Rikkunshito, one of these medicines, has not been evaluated with respect to its influence on gastrointestinal motor activity. We investigated the effect of rikkunshito on upper gastrointestinal motility and plasma ghrelin concentrations in conscious dogs. Methods: Contractile response to intragastric administration of rikkunshito was studied via surgically implanted force transducers. A powdered extract of rikkunshito (1.3, 2.7, and 4.0 g) dissolved in water was administered into the stomachs of normal and vagotomized dogs before feeding and gastric emptying was evaluated. Several inhibitors of gastrointestinal motility (atropine, hexamethonium, and ondansetron) were injected intravenously before intragastric administration of rikkunshito. Plasma acylated ghrelin levels after intragastric administration of rikkunshito were measured. Results: In a fasting state, intragastric administration of rikkunshito induced phasic contractions in the duodenum and jejunum in normal dogs. Rikkunshito-induced contractions were inhibited by atropine, hexamethonium and ondansetron. In vagotomized dogs, rikkunshito induced phasic contractions, similar to normal dogs. Gastric emptying was accelerated by intragastric administration of rikkunshito in a dose-dependent manner. The plasma acylated ghrelin level 150 min after intragastric administration of 4.0 g of rikkunshito was significantly higher than the control value. Conclusions: Intragastric administration of rikkunshito stimulates gastrointestinal contractions in the interdigestive state through cholinergic neurons and 5-HT type 3 receptors. Moreover, rikkunshito increases plasma acylated ghrelin levels. Rikkunshito may alleviate gastrointestinal disorders through its prokinetic effects.

AB - Background: Traditional Japanese medicine, known as Kampo medicine, consists of mixtures of several medicinal herbs widely used to treat upper gastrointestinal disorders in Japan. Rikkunshito, one of these medicines, has not been evaluated with respect to its influence on gastrointestinal motor activity. We investigated the effect of rikkunshito on upper gastrointestinal motility and plasma ghrelin concentrations in conscious dogs. Methods: Contractile response to intragastric administration of rikkunshito was studied via surgically implanted force transducers. A powdered extract of rikkunshito (1.3, 2.7, and 4.0 g) dissolved in water was administered into the stomachs of normal and vagotomized dogs before feeding and gastric emptying was evaluated. Several inhibitors of gastrointestinal motility (atropine, hexamethonium, and ondansetron) were injected intravenously before intragastric administration of rikkunshito. Plasma acylated ghrelin levels after intragastric administration of rikkunshito were measured. Results: In a fasting state, intragastric administration of rikkunshito induced phasic contractions in the duodenum and jejunum in normal dogs. Rikkunshito-induced contractions were inhibited by atropine, hexamethonium and ondansetron. In vagotomized dogs, rikkunshito induced phasic contractions, similar to normal dogs. Gastric emptying was accelerated by intragastric administration of rikkunshito in a dose-dependent manner. The plasma acylated ghrelin level 150 min after intragastric administration of 4.0 g of rikkunshito was significantly higher than the control value. Conclusions: Intragastric administration of rikkunshito stimulates gastrointestinal contractions in the interdigestive state through cholinergic neurons and 5-HT type 3 receptors. Moreover, rikkunshito increases plasma acylated ghrelin levels. Rikkunshito may alleviate gastrointestinal disorders through its prokinetic effects.

KW - Gastric emptying

KW - Gastrointestinal motility

KW - Ghrelin

KW - Rikkunshito

UR - http://www.scopus.com/inward/record.url?scp=84878109728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878109728&partnerID=8YFLogxK

U2 - 10.1007/s00535-012-0687-8

DO - 10.1007/s00535-012-0687-8

M3 - Article

C2 - 23053427

AN - SCOPUS:84878109728

VL - 48

SP - 611

EP - 619

JO - Journal of Gastroenterology

JF - Journal of Gastroenterology

SN - 0944-1174

IS - 5

ER -