Intranuclear ubiquitin immunoreactivity in the pigmented neurons of the substantia nigra in fire fatalities

L. Quan, B. L. Zhu, S. Oritani, K. Ishida, M. Q. Fujita, H. Maeda

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


To evaluate the significance of immunohistochemical staining of ubiquitin (heat shock protein) in the midbrain for medico-legal investigation of death in fires, we examined forensic autopsy cases of fire fatalities (n = 35) in comparison with controls (n = 27; brain stem injury, acute myocardial infarction and carbon monoxide poisoning other than fire fatality). There were two intranuclear staining patterns in the nuclei of pigmented substantia nigra neurons: a type of inclusion (possible Marinesco bodies) and a diffuse staining. Percentage of nuclear ubiquitin positivity (Ub-positive %) in fire fatalities (2.7-44.7%; mean, 18.5%) was significantly higher than in brain stem injury (n = 9; 0-10.4%; mean, 4.5%) and myocardial infarction (n = 14; 1.5-14.6%; mean, 6.9%), independently of blood carboxyhemoglobin (COHb) levels. Age-dependent increase in Ub-positive % was observed in lower COHb (< 60%) cases. The intranuclear diffuse ubiquitin staining was not observed in cases of high blood cyanide level (> 1.0 μg/ml). These observations showed that intranuclear ubiquitin immunoreactivity of the pigmented substantia nigra neurons in the midbrain was induced by severe stress in fires.

Original languageEnglish
Pages (from-to)310-315
Number of pages6
JournalInternational Journal of Legal Medicine
Issue number6
Publication statusPublished - 2001
Externally publishedYes


  • Fire fatality
  • Immunohistochemistry
  • Physical activity
  • Pigmented substantia nigra neurons
  • Ubiquitin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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