Intratracheal instillation of perfluorocarbon rescued mice with primary pulmonary hypoplasia

Kazuhiro Mori, Nobuyoshi Kurihara, Shinnosuke Obi, Shinya Hayashida, Keisuke Tokieda, Kazushige Ikeda

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the effect of perfluorocarbon on the respiratory status of newborn mice with pulmonary hypoplasia without diaphragmatic defects, following intrauterine exposure to nitrofen. Three groups of newborn mice were compared: pups exposed to nitrofen antenatally without (group A) or with (group B) perfluorocarbon treatment and pups not exposed to nitrofen as a control (group C). Respiratory evaluation was performed by scoring, pressure-volume analysis, and histological examination. At 40 minutes after birth, the survival rates in groups A, B, and C were 51%, 94%, and 95%, respectively. The clinical scores of group B mice at 40 minutes were significantly better than those of group A mice in which pulmonary hypoplasia was induced. In group B, the hysteresis ratio was significantly higher than that in group A, and lung histology showed a significant increase in airspace. An immunohistochemical examination showed that perfluorocarbon did not alter the expression of mature surfactant protein B and surfactant proprotein C. This study demonstrated that treatment with perfluorocarbon was useful in stabilizing critically ill mice with primary pulmonary hypoptasia during the early phase of therapy.

Original languageEnglish
Pages (from-to)523-533
Number of pages11
JournalExperimental Lung Research
Volume28
Issue number6
DOIs
Publication statusPublished - 2002 Aug 24

Keywords

  • Nitrofen
  • Partial liquid ventilation
  • Perfluorocarbon
  • Pulmonary hypoplasia
  • Surfactant protein

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

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