Abstract
Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.
Original language | English |
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Pages (from-to) | 145-153 |
Number of pages | 9 |
Journal | Methods in Molecular Biology |
Volume | 1139 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- BCG-CWS
- Cryoablation
- Dendritic cells
- Immunotherapy
- KLH
ASJC Scopus subject areas
- Molecular Biology
- Genetics