Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura; Masaru Udagawa; Tomonobu Fujita; Toshiharu Sakurai; Tomonori Yaguchi; Yutaka Kawakami

doi:10.1007/978-1-4939-0345-0_13

Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, Yutaka Kawakami

Division of Cellular Signaling

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Abstract

Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

Original language	English
Pages (from-to)	145-153
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1139
DOIs	https://doi.org/10.1007/978-1-4939-0345-0_13
Publication status	Published - 2014 Jan 1

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Keywords

BCG-CWS
Cryoablation
Dendritic cells
Immunotherapy
KLH

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Kawamura, N., Udagawa, M., Fujita, T., Sakurai, T., Yaguchi, T., & Kawakami, Y. (2014). Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation. Methods in Molecular Biology, 1139, 145-153. https://doi.org/10.1007/978-1-4939-0345-0_13

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10.1007/978-1-4939-0345-0_13