Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura; Masaru Udagawa; Tomonobu Fujita; Toshiharu Sakurai; Tomonori Yaguchi; Yutaka Kawakami

doi:10.1007/978-1-4939-0345-0_13

Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, Yutaka Kawakami

Division of Cellular Signaling

Research output: Contribution to journal › Article › peer-review

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Abstract

Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

Original language	English
Pages (from-to)	145-153
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1139
DOIs	https://doi.org/10.1007/978-1-4939-0345-0_13
Publication status	Published - 2014

Keywords

BCG-CWS
Cryoablation
Dendritic cells
Immunotherapy
KLH

ASJC Scopus subject areas

Molecular Biology
Genetics

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abstract = "Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.",

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author = "Naoshi Kawamura and Masaru Udagawa and Tomonobu Fujita and Toshiharu Sakurai and Tomonori Yaguchi and Yutaka Kawakami",

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AU - Kawamura, Naoshi

AU - Udagawa, Masaru

AU - Fujita, Tomonobu

AU - Sakurai, Toshiharu

AU - Yaguchi, Tomonori

AU - Kawakami, Yutaka

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

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Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Abstract

Keywords

ASJC Scopus subject areas

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