Abstract
Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.
| Original language | English |
|---|---|
| Pages (from-to) | 145-153 |
| Number of pages | 9 |
| Journal | Methods in Molecular Biology |
| Volume | 1139 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- BCG-CWS
- Cryoablation
- Dendritic cells
- Immunotherapy
- KLH
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Medicine(all)
Cite this
Intratumoral Injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation. / Kawamura, Naoshi; Udagawa, Masaru; Fujita, Tomonobu; Sakurai, Toshiharu; Yaguchi, Tomonori; Kawakami, Yutaka.
In: Methods in Molecular Biology, Vol. 1139, 2014, p. 145-153.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intratumoral Injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation
AU - Kawamura, Naoshi
AU - Udagawa, Masaru
AU - Fujita, Tomonobu
AU - Sakurai, Toshiharu
AU - Yaguchi, Tomonori
AU - Kawakami, Yutaka
PY - 2014
Y1 - 2014
N2 - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.
AB - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.
KW - BCG-CWS
KW - Cryoablation
KW - Dendritic cells
KW - Immunotherapy
KW - KLH
UR - http://www.scopus.com/inward/record.url?scp=84909636878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84909636878&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0345-0_13
DO - 10.1007/978-1-4939-0345-0_13
M3 - Article
C2 - 24619677
AN - SCOPUS:84909636878
VL - 1139
SP - 145
EP - 153
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -