Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation

Makoto Suematsu, Masaya Oda, Hidekazu Suzuki, Hiroshi Kaneko, Norihito Watanabe, Tadasu Furusho, Shoichi Masushige, Masaharu Tsuchiya

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Intralobular distribution of Ito cells (fat-storing cells) in hepatic microcirculatory units was investigated through intravital fluorescence microscopy. By using a low-level ultraviolet epi-illumination and a sensitive silicon intensified target camera, the intrahepatic autofluorescence was visualized and digitally processed. In rats fed an ordinary diet, the ultraviolet-excited autofluorescence was composed of at least two different origins that could not be spectrophotometrically distinguished, namely, multiple patchy autofluorescence activities along the sinusoids and terminal hepatic venules and diffuse parenchymal autofluorescence. Multiple patchy activities showed a rapid photobleaching phenomenon under the continuous ultraviolet excitation. These fluorescent activities were completely eliminated by depleting the intrahepatic retinoid contents using a vitamin A-deficient diet for 4 weeks. Furthermore repeated administration of vitamin A significantly enhanced the patchy fluorescent activities. Electron microscopy revealed that these vitamin A fluorescent activities are colocalized with the fat droplets in Ito cells, providing evidence that Ito cells exist not only in perisinusoidal spaces but also in the perivascular spaces of the terminal hepatic and portal venules. The current intravital technique thus provides a new method to observe Ito cells in hepatic microcirculatory units in vivo.

Original languageEnglish
Pages (from-to)28-42
Number of pages15
JournalMicrovascular Research
Volume46
Issue number1
DOIs
Publication statusPublished - 1993 Jul

Fingerprint

Microcirculation
Hepatic Stellate Cells
Rats
Vitamin A
Electrons
Venules
Liver
Nutrition
Fats
Diet
Photobleaching
Fluorescence microscopy
Retinoids
Silicon
Lighting
Fluorescence Microscopy
Adipocytes
Electron microscopy
Electron Microscopy
Cameras

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Biochemistry

Cite this

Suematsu, M., Oda, M., Suzuki, H., Kaneko, H., Watanabe, N., Furusho, T., ... Tsuchiya, M. (1993). Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation. Microvascular Research, 46(1), 28-42. https://doi.org/10.1006/mvre.1993.1033

Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation. / Suematsu, Makoto; Oda, Masaya; Suzuki, Hidekazu; Kaneko, Hiroshi; Watanabe, Norihito; Furusho, Tadasu; Masushige, Shoichi; Tsuchiya, Masaharu.

In: Microvascular Research, Vol. 46, No. 1, 07.1993, p. 28-42.

Research output: Contribution to journalArticle

Suematsu, M, Oda, M, Suzuki, H, Kaneko, H, Watanabe, N, Furusho, T, Masushige, S & Tsuchiya, M 1993, 'Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation', Microvascular Research, vol. 46, no. 1, pp. 28-42. https://doi.org/10.1006/mvre.1993.1033
Suematsu, Makoto ; Oda, Masaya ; Suzuki, Hidekazu ; Kaneko, Hiroshi ; Watanabe, Norihito ; Furusho, Tadasu ; Masushige, Shoichi ; Tsuchiya, Masaharu. / Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation. In: Microvascular Research. 1993 ; Vol. 46, No. 1. pp. 28-42.
@article{1e0a47142dc34549b41faa11a7a038c4,
title = "Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation",
abstract = "Intralobular distribution of Ito cells (fat-storing cells) in hepatic microcirculatory units was investigated through intravital fluorescence microscopy. By using a low-level ultraviolet epi-illumination and a sensitive silicon intensified target camera, the intrahepatic autofluorescence was visualized and digitally processed. In rats fed an ordinary diet, the ultraviolet-excited autofluorescence was composed of at least two different origins that could not be spectrophotometrically distinguished, namely, multiple patchy autofluorescence activities along the sinusoids and terminal hepatic venules and diffuse parenchymal autofluorescence. Multiple patchy activities showed a rapid photobleaching phenomenon under the continuous ultraviolet excitation. These fluorescent activities were completely eliminated by depleting the intrahepatic retinoid contents using a vitamin A-deficient diet for 4 weeks. Furthermore repeated administration of vitamin A significantly enhanced the patchy fluorescent activities. Electron microscopy revealed that these vitamin A fluorescent activities are colocalized with the fat droplets in Ito cells, providing evidence that Ito cells exist not only in perisinusoidal spaces but also in the perivascular spaces of the terminal hepatic and portal venules. The current intravital technique thus provides a new method to observe Ito cells in hepatic microcirculatory units in vivo.",
author = "Makoto Suematsu and Masaya Oda and Hidekazu Suzuki and Hiroshi Kaneko and Norihito Watanabe and Tadasu Furusho and Shoichi Masushige and Masaharu Tsuchiya",
year = "1993",
month = "7",
doi = "10.1006/mvre.1993.1033",
language = "English",
volume = "46",
pages = "28--42",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Intravital and Electron Microscopic Observation of Ito Cells in Rat Hepatic Microcirculation

AU - Suematsu, Makoto

AU - Oda, Masaya

AU - Suzuki, Hidekazu

AU - Kaneko, Hiroshi

AU - Watanabe, Norihito

AU - Furusho, Tadasu

AU - Masushige, Shoichi

AU - Tsuchiya, Masaharu

PY - 1993/7

Y1 - 1993/7

N2 - Intralobular distribution of Ito cells (fat-storing cells) in hepatic microcirculatory units was investigated through intravital fluorescence microscopy. By using a low-level ultraviolet epi-illumination and a sensitive silicon intensified target camera, the intrahepatic autofluorescence was visualized and digitally processed. In rats fed an ordinary diet, the ultraviolet-excited autofluorescence was composed of at least two different origins that could not be spectrophotometrically distinguished, namely, multiple patchy autofluorescence activities along the sinusoids and terminal hepatic venules and diffuse parenchymal autofluorescence. Multiple patchy activities showed a rapid photobleaching phenomenon under the continuous ultraviolet excitation. These fluorescent activities were completely eliminated by depleting the intrahepatic retinoid contents using a vitamin A-deficient diet for 4 weeks. Furthermore repeated administration of vitamin A significantly enhanced the patchy fluorescent activities. Electron microscopy revealed that these vitamin A fluorescent activities are colocalized with the fat droplets in Ito cells, providing evidence that Ito cells exist not only in perisinusoidal spaces but also in the perivascular spaces of the terminal hepatic and portal venules. The current intravital technique thus provides a new method to observe Ito cells in hepatic microcirculatory units in vivo.

AB - Intralobular distribution of Ito cells (fat-storing cells) in hepatic microcirculatory units was investigated through intravital fluorescence microscopy. By using a low-level ultraviolet epi-illumination and a sensitive silicon intensified target camera, the intrahepatic autofluorescence was visualized and digitally processed. In rats fed an ordinary diet, the ultraviolet-excited autofluorescence was composed of at least two different origins that could not be spectrophotometrically distinguished, namely, multiple patchy autofluorescence activities along the sinusoids and terminal hepatic venules and diffuse parenchymal autofluorescence. Multiple patchy activities showed a rapid photobleaching phenomenon under the continuous ultraviolet excitation. These fluorescent activities were completely eliminated by depleting the intrahepatic retinoid contents using a vitamin A-deficient diet for 4 weeks. Furthermore repeated administration of vitamin A significantly enhanced the patchy fluorescent activities. Electron microscopy revealed that these vitamin A fluorescent activities are colocalized with the fat droplets in Ito cells, providing evidence that Ito cells exist not only in perisinusoidal spaces but also in the perivascular spaces of the terminal hepatic and portal venules. The current intravital technique thus provides a new method to observe Ito cells in hepatic microcirculatory units in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0027295598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027295598&partnerID=8YFLogxK

U2 - 10.1006/mvre.1993.1033

DO - 10.1006/mvre.1993.1033

M3 - Article

C2 - 8412851

AN - SCOPUS:0027295598

VL - 46

SP - 28

EP - 42

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

IS - 1

ER -