Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches

Hitoshi Fujimori, Soichiro Miura, Seiichiro Koseki, Ryota Hokari, Yoshikazu Tsuzuki, Shunsuke Komoto, Yuriko Hara, Hidekazu Suzuki, Hiroshi Serizawa, Hiromasa Ishii

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background/Aims: Cytokine-induced neutrophil chemoattractant (CINC/gro), a member of interleukin-8 family, was found as a potent chemotactic factor for rat neutrophils. Although several chemokines have been shown to be potent regulators of T cell chemotaxis in vitro, the potential role of chemokines in T-cell migration in gut-associated lymphoid tissues has not been investigated in vivo. In the present study, the effects of CINC/gro on T-lymphocyte migration were examined in rat Peyer's patches. Methods: T lymphocytes collected from intestinal lymph of rats were fluorescence-labeled and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy and the effects of CINC/gro infusion was investigated. Lymphocyte flux in mesenteric collecting lymphatics was also observed. Results: In vivo infusion of CINC/gro significantly attenuated the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, once these lymphocytes adhered to venules, CINC/gro treatment significantly accelerated the transendothelial migration of T lymphocytes and they also significantly increased their subsequent flux in collecting lymphatics. Conclusion: There is a possibility that CINC/gro could modulate the characteristics of T lymphocyte homing in the inflammatory sites of gut.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalDigestion
Volume63
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2001

Fingerprint

Peyer's Patches
T-Lymphocytes
Venules
Lymphocytes
Interleukin-8
Chemokines
Transendothelial and Transepithelial Migration
Chemotactic Factors
Jugular Veins
Lymphoid Tissue
Lymph
Chemotaxis
Fluorescence Microscopy
Cell Movement
Neutrophils
Fluorescence
Cytokines

Keywords

  • CINC/gro
  • Collecting lymphatics
  • Peyer's patch
  • Postcapillary venules
  • T lymphocytes
  • Transendothelial migration

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Fujimori, H., Miura, S., Koseki, S., Hokari, R., Tsuzuki, Y., Komoto, S., ... Ishii, H. (2001). Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches. Digestion, 63(SUPPL. 1), 97-102. https://doi.org/10.1159/000051919

Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches. / Fujimori, Hitoshi; Miura, Soichiro; Koseki, Seiichiro; Hokari, Ryota; Tsuzuki, Yoshikazu; Komoto, Shunsuke; Hara, Yuriko; Suzuki, Hidekazu; Serizawa, Hiroshi; Ishii, Hiromasa.

In: Digestion, Vol. 63, No. SUPPL. 1, 2001, p. 97-102.

Research output: Contribution to journalArticle

Fujimori, H, Miura, S, Koseki, S, Hokari, R, Tsuzuki, Y, Komoto, S, Hara, Y, Suzuki, H, Serizawa, H & Ishii, H 2001, 'Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches', Digestion, vol. 63, no. SUPPL. 1, pp. 97-102. https://doi.org/10.1159/000051919
Fujimori H, Miura S, Koseki S, Hokari R, Tsuzuki Y, Komoto S et al. Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches. Digestion. 2001;63(SUPPL. 1):97-102. https://doi.org/10.1159/000051919
Fujimori, Hitoshi ; Miura, Soichiro ; Koseki, Seiichiro ; Hokari, Ryota ; Tsuzuki, Yoshikazu ; Komoto, Shunsuke ; Hara, Yuriko ; Suzuki, Hidekazu ; Serizawa, Hiroshi ; Ishii, Hiromasa. / Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches. In: Digestion. 2001 ; Vol. 63, No. SUPPL. 1. pp. 97-102.
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AU - Tsuzuki, Yoshikazu

AU - Komoto, Shunsuke

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