Intravital Two-photon Imaging of Ca2+ signaling in Secretory Organs of Yellow Cameleon Transgenic Mice

Kai Jin, Toshihiro Imada, Shigeru Nakamura, Yusuke Izuta, Erina Oonishi, Michiko Shibuya, Hisayo Sakaguchi, Takahiro Adachi, Kazuo Tsubota

Research output: Contribution to journalArticle

Abstract

Intracellular calcium ([Ca2+]i) signaling regulates physiological functions in most cells. In secretory organs, such as the pancreas, salivary gland, and lacrimal gland (LG), [Ca2+]i elevation in acinar cells triggers fluid secretion, which plays vital roles in the maintenance of functional health across the life-course. It is important to understand the secretory mechanism of secretory organs, but lack of analytic systems available for living animals limits the scope of research to gain deeper insights into the precise mechanism of secretion. We established an intravital imaging system for specific cell types of secretory organs to monitor the [Ca2+]i changes using mouse line expressing Yellow Cameleon 3.60, a genetically encoded Ca2+ indicator. Elevation of [Ca2+]i in specific cell types of secretory organs could be monitored after cholinergic stimulation ex vivo and intravitally. We found that a marked attenuation of LG [Ca2+]i response to cholinergic stimulation was induced under pathological conditions by postganglionic denervation. Intravital Ca2+ imaging in secretory organs will broaden our understanding of the cellular mechanisms in animal models of secretory diseases.

Original languageEnglish
Article number15880
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Photons
Transgenic Mice
Lacrimal Apparatus
Cholinergic Agents
Fluids and Secretions
Animal Disease Models
Acinar Cells
Denervation
Salivary Glands
Pancreas
Calcium
Health
Research
yellow cameleon
Intravital Microscopy

ASJC Scopus subject areas

  • General

Cite this

Intravital Two-photon Imaging of Ca2+ signaling in Secretory Organs of Yellow Cameleon Transgenic Mice. / Jin, Kai; Imada, Toshihiro; Nakamura, Shigeru; Izuta, Yusuke; Oonishi, Erina; Shibuya, Michiko; Sakaguchi, Hisayo; Adachi, Takahiro; Tsubota, Kazuo.

In: Scientific Reports, Vol. 8, No. 1, 15880, 01.12.2018.

Research output: Contribution to journalArticle

Jin, K, Imada, T, Nakamura, S, Izuta, Y, Oonishi, E, Shibuya, M, Sakaguchi, H, Adachi, T & Tsubota, K 2018, 'Intravital Two-photon Imaging of Ca2+ signaling in Secretory Organs of Yellow Cameleon Transgenic Mice', Scientific Reports, vol. 8, no. 1, 15880. https://doi.org/10.1038/s41598-018-34347-1
Jin, Kai ; Imada, Toshihiro ; Nakamura, Shigeru ; Izuta, Yusuke ; Oonishi, Erina ; Shibuya, Michiko ; Sakaguchi, Hisayo ; Adachi, Takahiro ; Tsubota, Kazuo. / Intravital Two-photon Imaging of Ca2+ signaling in Secretory Organs of Yellow Cameleon Transgenic Mice. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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