Aims: The mechanism of the host cell invasion of Plesiomonas shigelloides and its capability to induce apoptosis were investigated. Methods and Results: We performed a time course experiment on the bacterial adherence and invasion of the P. shigelloides P-1 strain into Caco-2 cells using an invasion assay and flow cytometry. The adherence of P. shigelloides to the Caco-2 cells was almost completed within 10 min after the infection. Thereafter, P. shigelloides starts internalization within the Caco-2 cells, which was completed within 60 min after the infection. Based on the invasion assay using nocodazole, cytochalasin D, and genistein, it became clear that the mechanism of the internalization depended on the signal transduction followed by the rearrangement of the cytoskeletal protein. Based on the DNA laddering and TUNEL methods, the cytotoxicity of the Caco-2 cells by the invasion of P. shigelloides occurred through the induction of apoptosis. Conclusions: This work demonstrated that the mechanism of invasion of P. shigelloides into Caco-2 cells and the invasion of P. shigelloides induces apoptotic cell death. Significance and Impact of the Study: This work revealed the virulence factor, which may be important for understanding of the pathogenesis of P. shigelloides.
- Flow cytometry
- Plesiomonas shigelloides
- Virulence factor
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology