Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Yoichiro Ijima, Kaori Matoishi, Yosuke Terao, Nobuhide Doi, Hiroshi Yanagawa, Hiromichi Ohta

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

Original languageEnglish
Pages (from-to)877-879
Number of pages3
JournalChemical Communications
Issue number7
DOIs
Publication statusPublished - 2005 Feb 21

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Mutagenesis
Enantioselectivity
malonate decarboxylase
Decarboxylation

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism. / Ijima, Yoichiro; Matoishi, Kaori; Terao, Yosuke; Doi, Nobuhide; Yanagawa, Hiroshi; Ohta, Hiromichi.

In: Chemical Communications, No. 7, 21.02.2005, p. 877-879.

Research output: Contribution to journalArticle

Ijima, Yoichiro ; Matoishi, Kaori ; Terao, Yosuke ; Doi, Nobuhide ; Yanagawa, Hiroshi ; Ohta, Hiromichi. / Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism. In: Chemical Communications. 2005 ; No. 7. pp. 877-879.
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