Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Yoichiro Ijima; Kaori Matoishi; Yosuke Terao; Nobuhide Doi; Hiroshi Yanagawa; Hiromichi Ohta

doi:10.1039/b416398b

Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Yoichiro Ijima, Kaori Matoishi, Yosuke Terao, Nobuhide Doi, Hiroshi Yanagawa, Hiromichi Ohta

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

Original language	English
Pages (from-to)	877-879
Number of pages	3
Journal	Chemical Communications
Issue number	7
DOIs	https://doi.org/10.1039/b416398b
Publication status	Published - 2005 Feb 21

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/b416398b

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title = "Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism",

abstract = "The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.",

author = "Yoichiro Ijima and Kaori Matoishi and Yosuke Terao and Nobuhide Doi and Hiroshi Yanagawa and Hiromichi Ohta",

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AU - Ijima, Yoichiro

AU - Matoishi, Kaori

AU - Terao, Yosuke

AU - Doi, Nobuhide

AU - Yanagawa, Hiroshi

AU - Ohta, Hiromichi

PY - 2005/2/21

Y1 - 2005/2/21

N2 - The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

AB - The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Abstract

ASJC Scopus subject areas

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