Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex

Michihiko Luca Waki, Kenji Onoue, Tsukasa Takahashi, Kensuke Goto, Yusuke Saito, Katsuaki Inami, Ippei Makita, Yurika Angata, Tomomi Suzuki, Mihi Yamashita, Narumi Sato, Saki Nakamura, Dai Yuki, Yuki Sugiura, Nobuhiro Zaima, Naoko Goto-Inoue, Takahiro Hayasaka, Yutaka Shimomura, Mitsutoshi Setou

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. Methods: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. Results: Among the 31 molecules detected specifically in hair sections, 2-one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)-exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. Conclusion: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution.

Original languageEnglish
Article numbere26721
JournalPLoS One
Volume6
Issue number10
DOIs
Publication statusPublished - 2011 Oct 24
Externally publishedYes

Fingerprint

Biomarkers
Hair
hairs
Mass spectrometry
biomarkers
Mass Spectrometry
cortex
Aging of materials
image analysis
mass spectrometry
Imaging techniques
Molecules
Cosmetics
Biomolecules
Ions
Tissue
cosmetics
Ionization
Desorption
ions

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Waki, M. L., Onoue, K., Takahashi, T., Goto, K., Saito, Y., Inami, K., ... Setou, M. (2011). Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex. PLoS One, 6(10), [e26721]. https://doi.org/10.1371/journal.pone.0026721

Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex. / Waki, Michihiko Luca; Onoue, Kenji; Takahashi, Tsukasa; Goto, Kensuke; Saito, Yusuke; Inami, Katsuaki; Makita, Ippei; Angata, Yurika; Suzuki, Tomomi; Yamashita, Mihi; Sato, Narumi; Nakamura, Saki; Yuki, Dai; Sugiura, Yuki; Zaima, Nobuhiro; Goto-Inoue, Naoko; Hayasaka, Takahiro; Shimomura, Yutaka; Setou, Mitsutoshi.

In: PLoS One, Vol. 6, No. 10, e26721, 24.10.2011.

Research output: Contribution to journalArticle

Waki, ML, Onoue, K, Takahashi, T, Goto, K, Saito, Y, Inami, K, Makita, I, Angata, Y, Suzuki, T, Yamashita, M, Sato, N, Nakamura, S, Yuki, D, Sugiura, Y, Zaima, N, Goto-Inoue, N, Hayasaka, T, Shimomura, Y & Setou, M 2011, 'Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex', PLoS One, vol. 6, no. 10, e26721. https://doi.org/10.1371/journal.pone.0026721
Waki, Michihiko Luca ; Onoue, Kenji ; Takahashi, Tsukasa ; Goto, Kensuke ; Saito, Yusuke ; Inami, Katsuaki ; Makita, Ippei ; Angata, Yurika ; Suzuki, Tomomi ; Yamashita, Mihi ; Sato, Narumi ; Nakamura, Saki ; Yuki, Dai ; Sugiura, Yuki ; Zaima, Nobuhiro ; Goto-Inoue, Naoko ; Hayasaka, Takahiro ; Shimomura, Yutaka ; Setou, Mitsutoshi. / Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex. In: PLoS One. 2011 ; Vol. 6, No. 10.
@article{3f313e62a9904300a648a192016c48e7,
title = "Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex",
abstract = "Background: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. Methods: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. Results: Among the 31 molecules detected specifically in hair sections, 2-one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)-exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. Conclusion: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution.",
author = "Waki, {Michihiko Luca} and Kenji Onoue and Tsukasa Takahashi and Kensuke Goto and Yusuke Saito and Katsuaki Inami and Ippei Makita and Yurika Angata and Tomomi Suzuki and Mihi Yamashita and Narumi Sato and Saki Nakamura and Dai Yuki and Yuki Sugiura and Nobuhiro Zaima and Naoko Goto-Inoue and Takahiro Hayasaka and Yutaka Shimomura and Mitsutoshi Setou",
year = "2011",
month = "10",
day = "24",
doi = "10.1371/journal.pone.0026721",
language = "English",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

TY - JOUR

T1 - Investigation by imaging mass spectrometry of biomarker candidates for aging in the hair cortex

AU - Waki, Michihiko Luca

AU - Onoue, Kenji

AU - Takahashi, Tsukasa

AU - Goto, Kensuke

AU - Saito, Yusuke

AU - Inami, Katsuaki

AU - Makita, Ippei

AU - Angata, Yurika

AU - Suzuki, Tomomi

AU - Yamashita, Mihi

AU - Sato, Narumi

AU - Nakamura, Saki

AU - Yuki, Dai

AU - Sugiura, Yuki

AU - Zaima, Nobuhiro

AU - Goto-Inoue, Naoko

AU - Hayasaka, Takahiro

AU - Shimomura, Yutaka

AU - Setou, Mitsutoshi

PY - 2011/10/24

Y1 - 2011/10/24

N2 - Background: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. Methods: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. Results: Among the 31 molecules detected specifically in hair sections, 2-one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)-exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. Conclusion: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution.

AB - Background: Human hair is one of the essential components that define appearance and is a useful source of samples for non-invasive biomonitoring. We describe a novel application of imaging mass spectrometry (IMS) of hair biomolecules for advanced molecular characterization and a better understanding of hair aging. As a cosmetic and biomedical application, molecules whose levels in hair altered with aging were comprehensively investigated. Methods: Human hair was collected from 15 young (20±5 years old) and 15 older (50±5 years old) volunteers. Matrix-free laser desorption/ionization IMS was used to visualize molecular distribution in the hair sections. Hair-specific ions displaying a significant difference in the intensities between the 2 age groups were extracted as candidate markers for aging. Tissue localization of the molecules and alterations in their levels in the cortex and medulla in the young and old groups were determined. Results: Among the 31 molecules detected specifically in hair sections, 2-one at m/z 153.00, tentatively assigned to be dihydrouracil, and the other at m/z 207.04, identified to be 3,4-dihydroxymandelic acid (DHMA)-exhibited a higher signal intensity in the young group than in the old, and 1 molecule at m/z 164.00, presumed to be O-phosphoethanolamine, displayed a higher intensity in the old group. Among the 3, putative O-phosphoethanolamine showed a cortex-specific distribution. The 3 molecules in cortex presented the same pattern of alteration in signal intensity with aging, whereas those in medulla did not exhibit significant alteration. Conclusion: Three molecules whose levels in hair altered with age were extracted. While they are all possible markers for aging, putative dihydrouracil and DHMA, are also suspected to play a role in maintaining hair properties and could be targets for cosmetic supplementation. Mapping of ion localization in hair by IMS is a powerful method to extract biomolecules in specified regions and determine their tissue distribution.

UR - http://www.scopus.com/inward/record.url?scp=80055049927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80055049927&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0026721

DO - 10.1371/journal.pone.0026721

M3 - Article

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 10

M1 - e26721

ER -