Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging

Takeshi Kubota, Yutaka Shindo, Kotaro Oka, Hirokazu Komatsu, Hiroto Ogawa, Yoshiichiro Kitamura, Koji Suzuki, Kotaro Oka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective and Methods: PC12 cells were loaded with a novel Mg indicator KMG-104 and Ca indicator fura-2, and intracellular Mg was studied in the endoplasmic reticulums (ERs), mitochondria, and Mg-ATP. Under coexistence of the two indicators, fluorescent signals of Mg and Ca can be measured separately. Mg release from the ER was investigated by photolysis of caged compounds. Results: Transient [Ca]i increase by uncaging of caged Ca or caged IP 3 or bath-application of caffeine (10 mM) induced no [Mg]i increase. These results suggest that there is no mechanism for Mg release from the ER through ryanodine receptors or IP3 receptors. In order to investigate the possibility of Mg release from Mg-ATP by energy consumption, we depleted ATP by oligomycin, an inhibitor of mitochondrial ATP synthase. Treating with oligomycin (4 μM) for several minutes showed no change of [Mg] i and [Ca]i. Conclusions: This result shows that Mg-ATP is not a Mg store. Since, when cells were treated by an uncoupler FCCP (3 μM), [Mg]i and [Ca]i increased, we concluded that mitochondria participate in maintenance of intracellular Mg stores.

Original languageEnglish
JournalJournal of the American College of Nutrition
Volume23
Issue number6
Publication statusPublished - 2004 Dec

Fingerprint

PC12 Cells
endoplasmic reticulum
Magnesium
oligomycin
B-lymphocytes
magnesium
Adenosine Triphosphate
image analysis
Endoplasmic Reticulum
Oligomycins
mitochondria
ryanodine receptors
Mitochondria
H-transporting ATP synthase
Mitochondrial Proton-Translocating ATPases
photolysis
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
caffeine
Inositol 1,4,5-Trisphosphate Receptors
Ryanodine Receptor Calcium Release Channel

Keywords

  • ATP
  • FCCP
  • Intracellular Mg store
  • Mitochondria
  • Photolysis

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging. / Kubota, Takeshi; Shindo, Yutaka; Oka, Kotaro; Komatsu, Hirokazu; Ogawa, Hiroto; Kitamura, Yoshiichiro; Suzuki, Koji; Oka, Kotaro.

In: Journal of the American College of Nutrition, Vol. 23, No. 6, 12.2004.

Research output: Contribution to journalArticle

Kubota, Takeshi ; Shindo, Yutaka ; Oka, Kotaro ; Komatsu, Hirokazu ; Ogawa, Hiroto ; Kitamura, Yoshiichiro ; Suzuki, Koji ; Oka, Kotaro. / Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging. In: Journal of the American College of Nutrition. 2004 ; Vol. 23, No. 6.
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