Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development

Mitsutoshi Yamada, Toshio Hamatani, Hidenori Akutsu, Nana Chikazawa, Naoaki Kuji, Yasunori Yoshimura, Akihiro Umezawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mining gene-expression-profiling data identified a novel gene that is specifically expressed in preimplantation embryos. Hmgpi, a putative chromosomal protein with two high-mobility-group boxes, is zygotically transcribed during zygotic genome activation, but is not transcribed postimplantation. The Hmgpi-encoded protein (HMGPI), first detected at the 4-cell stage, remains highly expressed in pre-implantation embryos. Interestingly, HMGPI is expressed in both the inner cell mass (ICM) and the trophectoderm, and translocated from cytoplasm to nuclei at the blastocyst stage, indicating differential spatial requirements before and after the blastocyst stage. siRNA (siHmgpi)-induced reduction of Hmgpi transcript levels caused developmental loss of preimplantation embryos and implantation failures. Furthermore, reduction of Hmgpi prevented blastocyst outgrowth leading to generation of embryonic stem cells. The siHmgpi-injected embryos also lost ICM and trophectoderm integrity, demarcated by reduced expressions of Oct4, Nanog and Cdx2. The findings implicated an important role for Hmgpi at the earliest stages of mammalian embryonic development.

Original languageEnglish
Article numberddp512
Pages (from-to)480-493
Number of pages14
JournalHuman Molecular Genetics
Volume19
Issue number3
DOIs
Publication statusPublished - 2009 Nov 14

Fingerprint

HMGB Proteins
Blastocyst
Embryonic Development
Genes
Gene Expression Profiling
Embryonic Stem Cells
Small Interfering RNA
Cytoplasm
Proteins
Embryonic Structures
Genome

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development. / Yamada, Mitsutoshi; Hamatani, Toshio; Akutsu, Hidenori; Chikazawa, Nana; Kuji, Naoaki; Yoshimura, Yasunori; Umezawa, Akihiro.

In: Human Molecular Genetics, Vol. 19, No. 3, ddp512, 14.11.2009, p. 480-493.

Research output: Contribution to journalArticle

Yamada, Mitsutoshi ; Hamatani, Toshio ; Akutsu, Hidenori ; Chikazawa, Nana ; Kuji, Naoaki ; Yoshimura, Yasunori ; Umezawa, Akihiro. / Involvement of a novel preimplantation-specific gene encoding the high mobility group box protein Hmgpi in early embryonic development. In: Human Molecular Genetics. 2009 ; Vol. 19, No. 3. pp. 480-493.
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