Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system

Akiko Joo Okumura, Kiyotaka Hatsuzawa, Taku Tamura, Hisao Nagaya, Kazuko Saeki, Fumihiko Okumura, Kenji Nagao, Mitsuo Nishikawa, Akihiko Yoshimura, Ikuo Wada

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The cellular endomembrane system requires the proper kinetic balance of synthesis and degradation of its individual components, which is maintained in part by a specific membrane fusion apparatus. In this study, we describe the molecular properties of D12, which was identified from a mouse expression library. This C-terminal anchored membrane protein has sequence similarity to both a yeast soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE), Use1p/Slt1p, and a recently identified human syntaxin 18-binding protein, p31. D12 formed a tight complex with syntaxin 18 as well as Sec22b and bound to α-SNAP, indicating that D12 is a SNARE protein. Although the majority of D12 is located in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartments at steady state, overexpression or knockdown of D12 had no obvious effects on membrane trafficking in the early secretory pathway. However, suppression of D12 expression caused rapid appearance of lipofuscin granules, accompanied by apoptotic cell death without the apparent activation of the unfolded protein response. The typical cause of lipofuscin formation is the impaired degradation of mitochondria by lysosomal degradative enzymes, and, consistent with this, we found that proper post-Golgi maturation of cathepsin D was impaired in D12-deficient cells. This unexpected observation was supported by evidence that D12 associates with VAMP7, a SNARE in the endosomallysosomal pathway. Hence, we suggest that D12 participates in the degradative function of lysosomes.

Original languageEnglish
Pages (from-to)4495-4506
Number of pages12
JournalJournal of Biological Chemistry
Volume281
Issue number7
DOIs
Publication statusPublished - 2006 Feb 17
Externally publishedYes

Fingerprint

SNARE Proteins
Quality Control
Qa-SNARE Proteins
Quality control
Lipofuscin
Endoplasmic Reticulum
Membranes
Unfolded Protein Response
Degradation
Cathepsin D
Proteins
Membrane Fusion
Mitochondria
Fungal Proteins
Secretory Pathway
Cell death
Lysosomes
Yeast
Carrier Proteins
Membrane Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Okumura, A. J., Hatsuzawa, K., Tamura, T., Nagaya, H., Saeki, K., Okumura, F., ... Wada, I. (2006). Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system. Journal of Biological Chemistry, 281(7), 4495-4506. https://doi.org/10.1074/jbc.M509715200

Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system. / Okumura, Akiko Joo; Hatsuzawa, Kiyotaka; Tamura, Taku; Nagaya, Hisao; Saeki, Kazuko; Okumura, Fumihiko; Nagao, Kenji; Nishikawa, Mitsuo; Yoshimura, Akihiko; Wada, Ikuo.

In: Journal of Biological Chemistry, Vol. 281, No. 7, 17.02.2006, p. 4495-4506.

Research output: Contribution to journalArticle

Okumura, AJ, Hatsuzawa, K, Tamura, T, Nagaya, H, Saeki, K, Okumura, F, Nagao, K, Nishikawa, M, Yoshimura, A & Wada, I 2006, 'Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system', Journal of Biological Chemistry, vol. 281, no. 7, pp. 4495-4506. https://doi.org/10.1074/jbc.M509715200
Okumura AJ, Hatsuzawa K, Tamura T, Nagaya H, Saeki K, Okumura F et al. Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system. Journal of Biological Chemistry. 2006 Feb 17;281(7):4495-4506. https://doi.org/10.1074/jbc.M509715200
Okumura, Akiko Joo ; Hatsuzawa, Kiyotaka ; Tamura, Taku ; Nagaya, Hisao ; Saeki, Kazuko ; Okumura, Fumihiko ; Nagao, Kenji ; Nishikawa, Mitsuo ; Yoshimura, Akihiko ; Wada, Ikuo. / Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 7. pp. 4495-4506.
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