Involvement of c-Src in carcinoma cell motility and metastasis

M. Sakamoto, M. Takamura, Y. Ino, A. Miura, T. Genda, S. Hirohashi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Carcinoma cells exhibit dysfunction/dysregulation of cell adhesion systems that correlates with their abilities to migrate, invade, and metastasize. Here we show that the tyrosine kinase c-Src is required for motility and metastasis of two carcinoma cell lines. Adherent KYN-2 cells having a high level of c-Src kinase activity become scattered, extend lamellipodia, and exhibit high motility. Expression of a dominant-negative mutant form of c-Src caused formation of stress fibers and focal adhesions, and markedly reduced motility. HCT15 cells extended lamellipodia and became scattered in response to lysophosphatidic acid stimulation in parallel with transient activation of c-Src, which was inhibited by expression of a dominant-negative mutant form of c-Src or treatment with a specific Src kinase inhibitor. Furthermore, implantation of dominant-negative c-Src transfectants into the peritoneal cavity of SCID mice resulted in reduced peritoneal dissemination compared with control transfectants. These findings indicate that c-Src activation is critically involved in carcinoma cell migration and metastasis.

Original languageEnglish
Pages (from-to)941-946
Number of pages6
JournalJapanese Journal of Cancer Research
Volume92
Issue number9
DOIs
Publication statusPublished - 2001 Jan 1
Externally publishedYes

Keywords

  • Cell motility
  • Metastasis
  • Rho
  • c-Src

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Involvement of c-Src in carcinoma cell motility and metastasis'. Together they form a unique fingerprint.

  • Cite this