Involvement of catalytic amino acid residues in enzyme-catalyzed polymerization for the synthesis of polyesters

Y. Suzuki, S. Taguchi, T. Saito, Kazunobu Toshima, S. Matsumura, Y. Doi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Recently, a variety of aliphatic polyesters have been synthesized using hydrolases such as lipases and PHB depolymerases, and the reaction mechanism for these enzyme-catalyzed polymerization has been discussed. In this paper, we have studied the involvement of the catalytic amino acid residues of the hydrolase in enzyme-catalyzed polymerization with an extracellular PHB depolymerase from Alcaligenes faecalis T1. A wild-type PHB depolymerase and three kinds of site-specific mutants (catalytic amino acids were substituted) were prepared and their polymerization activities for the ring-opening polymerization of (R)-β-butyrolactone (BL) were compared. BL was polymerized at 80 °C in bulk by the wild-type enzyme to yield polymers consisting of cyclic and linear structures in a high monomer conversion. In contrast, none of the mutant enzymes showed obvious polymerization activity. These results have clearly demonstrated that the catalytic triad is indeed responsible for the enzyme-catalyzed polymerization of BL.

Original languageEnglish
Pages (from-to)541-544
Number of pages4
JournalBiomacromolecules
Volume2
Issue number2
DOIs
Publication statusPublished - 2001

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Polyesters
Polymerization
Amino acids
Enzymes
Amino Acids
Hydrolases
Alcaligenes faecalis
Ring opening polymerization
Lipases
Lipase
Polymers
Monomers
poly-beta-hydroxybutyrate depolymerase

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Involvement of catalytic amino acid residues in enzyme-catalyzed polymerization for the synthesis of polyesters. / Suzuki, Y.; Taguchi, S.; Saito, T.; Toshima, Kazunobu; Matsumura, S.; Doi, Y.

In: Biomacromolecules, Vol. 2, No. 2, 2001, p. 541-544.

Research output: Contribution to journalArticle

Suzuki, Y. ; Taguchi, S. ; Saito, T. ; Toshima, Kazunobu ; Matsumura, S. ; Doi, Y. / Involvement of catalytic amino acid residues in enzyme-catalyzed polymerization for the synthesis of polyesters. In: Biomacromolecules. 2001 ; Vol. 2, No. 2. pp. 541-544.
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