Involvement of dpy19l3 in myogenic differentiation of c2c12 myoblasts

Kento Mori, Hongkai Sun, Kazuki Miura, Siro Simizu

Research output: Contribution to journalArticlepeer-review

Abstract

DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

Original languageEnglish
Article number5685
JournalMolecules
Volume26
Issue number18
DOIs
Publication statusPublished - 2021 Sep

Keywords

  • C-mannosylation
  • DPY19L3
  • Glycobiology
  • Myogenic differentiation
  • Myotube

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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