Involvement of E-cadherin cleavage in reperfusion injury

Taichiro Goto, Akitoshi Ishizaka, Masahiko Katayama, Mitsutomo Kohno, Sadatomo Tasaka, Seitaro Fujishima, Koichi Kobayashi, Hiroaki Nomori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: E-cadherin is a major cell-to-cell adhesion molecule, of which the ectodomain is cleaved from epithelial cells to yield a soluble form after the pathological alteration of the alveolar epithelium. We investigated the excretion level of soluble E-cadherin in a rat lung isotransplant model, and demonstrated the involvement of this molecule in the pathogenesis of reperfusion injury after lung transplantation. Methods: Inbred male Lewis rats were used as both donor and recipient animals, and they were subjected to left lung isotransplantation. After 6 h of ischaemia, the left lung was transplanted into a recipient rat and reperfused for 4 h. The animals were injected intravenously with 125I-labelled albumin at 3 h after the onset of reperfusion as a marker of pulmonary albumin leakage. We assessed pulmonary alveolar septal damage quantitatively based on the 125I-albumin concentration ratio of bronchoalveolar lavage fluid (BALF) to plasma. Soluble E-cadherin fragments were detected in BALF on Western blot analysis using affinity-purified antibodies specific to rat E-cadherin synthetic peptides. Results: The BALF supernatant-to-plasma ratio of the graft lung was significantly increased compared to that of the control group. Western blot analysis showed a marked release of soluble E-cadherin into BALF, and its increase in BALF was associated with alveolar septal damage. Conclusions: These results suggest that one potential mechanism of lung reperfusion injury involves the cleavage of E-cadherin.

Original languageEnglish
Pages (from-to)426-431
Number of pages6
JournalEuropean Journal of Cardio-thoracic Surgery
Volume37
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

Keywords

  • E-cadherin
  • Ectodomain shedding
  • Lung transplantation
  • Reperfusion injury
  • Soluble form

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Goto, T., Ishizaka, A., Katayama, M., Kohno, M., Tasaka, S., Fujishima, S., Kobayashi, K., & Nomori, H. (2010). Involvement of E-cadherin cleavage in reperfusion injury. European Journal of Cardio-thoracic Surgery, 37(2), 426-431. https://doi.org/10.1016/j.ejcts.2009.06.041