Involvement of Ext1 and heparanase in migration of mouse FBJ osteosarcoma cells

Yinan Wang; Xiaoyan Yang; Sadako Yamagata; Tatsuya Yamagata; Toshinori Sato

doi:10.1007/s11010-012-1475-8

Involvement of Ext1 and heparanase in migration of mouse FBJ osteosarcoma cells

Yinan Wang, Xiaoyan Yang, Sadako Yamagata, Tatsuya Yamagata, Toshinori Sato

Department of Biosciences and Informatics

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

To know the involvement of glycosaminoglycans (GAGs) in the metastasis of mouse FBJ osteosarcoma cells, N ^α -lauroyl-O-(β-d- xylopyranosyl)-l-serinamide (Xyl-Ser-C12), which initiates elongation of GAG chains using the glycan biosynthesis system in cells, was administered to FBJ cells with different metastatic capacities. Production of glycosylated products derived from Xyl-Ser-C12, especially heparan sulfate (HS) GAG-type oligosaccharides such as GalNAc-GlcA-GlcNAc-GlcA-Gal-Gal-Xyl-Ser-C12, was indicated in poorly metastatic FBJ-S1 cells more than in highly metastatic FBJ-LL cells by LC-MS. The results of RT-PCR revealed that HS synthases, Ext1 and Ext2, were expressed in FBJ-S1 cells more than in FBJ-LL cells. Furthermore, siRNA against Ext1 suppressed the expression of HS and enhanced the motility of FBJ-S1 cells. In addition, the expression of heparanase (HPSE) was enhanced in Ext-1-knockdown FBJ-S1 cells, and responsible for the increase in cell motility caused by the down-regulation of Ext1 expression. Our data provide the first evidence that Ext1 regulates the expression of HPSE and also indicated that levels of Ext1 and HPSE influenced the motility of FBJ cells.

Original language	English
Pages (from-to)	63-72
Number of pages	10
Journal	Molecular and Cellular Biochemistry
Volume	373
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-012-1475-8
Publication status	Published - 2013 Jan

Keywords

Cell motility
Ext1
FBJ osteosarcoma
Glycosaminoglycan
Heparan sulfate
Heparanase
Saccharide primer

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/s11010-012-1475-8

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@article{14c973380c624f07aa320dcfdf6f3c31,

title = "Involvement of Ext1 and heparanase in migration of mouse FBJ osteosarcoma cells",

abstract = "To know the involvement of glycosaminoglycans (GAGs) in the metastasis of mouse FBJ osteosarcoma cells, N α -lauroyl-O-(β-d- xylopyranosyl)-l-serinamide (Xyl-Ser-C12), which initiates elongation of GAG chains using the glycan biosynthesis system in cells, was administered to FBJ cells with different metastatic capacities. Production of glycosylated products derived from Xyl-Ser-C12, especially heparan sulfate (HS) GAG-type oligosaccharides such as GalNAc-GlcA-GlcNAc-GlcA-Gal-Gal-Xyl-Ser-C12, was indicated in poorly metastatic FBJ-S1 cells more than in highly metastatic FBJ-LL cells by LC-MS. The results of RT-PCR revealed that HS synthases, Ext1 and Ext2, were expressed in FBJ-S1 cells more than in FBJ-LL cells. Furthermore, siRNA against Ext1 suppressed the expression of HS and enhanced the motility of FBJ-S1 cells. In addition, the expression of heparanase (HPSE) was enhanced in Ext-1-knockdown FBJ-S1 cells, and responsible for the increase in cell motility caused by the down-regulation of Ext1 expression. Our data provide the first evidence that Ext1 regulates the expression of HPSE and also indicated that levels of Ext1 and HPSE influenced the motility of FBJ cells.",

keywords = "Cell motility, Ext1, FBJ osteosarcoma, Glycosaminoglycan, Heparan sulfate, Heparanase, Saccharide primer",

author = "Yinan Wang and Xiaoyan Yang and Sadako Yamagata and Tatsuya Yamagata and Toshinori Sato",

note = "Funding Information: Acknowledgments This study was partly supported by the Global COE Project {\textquoteleft}{\textquoteleft}Global COE for Human Metabolomic Systems Biology{\textquoteright}{\textquoteright} (YW) from the Ministry of Education, Culture, Sports, Science and Technology and a Grant-in-aid for Challenging and Exploratory Research (2265108, TS) from the Japan Society for the Promotion of Science.",

year = "2013",

month = jan,

doi = "10.1007/s11010-012-1475-8",

language = "English",

volume = "373",

pages = "63--72",

journal = "Enzymologia",

issn = "0300-8177",

publisher = "Springer Netherlands",

number = "1-2",

}

TY - JOUR

T1 - Involvement of Ext1 and heparanase in migration of mouse FBJ osteosarcoma cells

AU - Wang, Yinan

AU - Yang, Xiaoyan

AU - Yamagata, Sadako

AU - Yamagata, Tatsuya

AU - Sato, Toshinori

N1 - Funding Information: Acknowledgments This study was partly supported by the Global COE Project ‘‘Global COE for Human Metabolomic Systems Biology’’ (YW) from the Ministry of Education, Culture, Sports, Science and Technology and a Grant-in-aid for Challenging and Exploratory Research (2265108, TS) from the Japan Society for the Promotion of Science.

PY - 2013/1

Y1 - 2013/1

N2 - To know the involvement of glycosaminoglycans (GAGs) in the metastasis of mouse FBJ osteosarcoma cells, N α -lauroyl-O-(β-d- xylopyranosyl)-l-serinamide (Xyl-Ser-C12), which initiates elongation of GAG chains using the glycan biosynthesis system in cells, was administered to FBJ cells with different metastatic capacities. Production of glycosylated products derived from Xyl-Ser-C12, especially heparan sulfate (HS) GAG-type oligosaccharides such as GalNAc-GlcA-GlcNAc-GlcA-Gal-Gal-Xyl-Ser-C12, was indicated in poorly metastatic FBJ-S1 cells more than in highly metastatic FBJ-LL cells by LC-MS. The results of RT-PCR revealed that HS synthases, Ext1 and Ext2, were expressed in FBJ-S1 cells more than in FBJ-LL cells. Furthermore, siRNA against Ext1 suppressed the expression of HS and enhanced the motility of FBJ-S1 cells. In addition, the expression of heparanase (HPSE) was enhanced in Ext-1-knockdown FBJ-S1 cells, and responsible for the increase in cell motility caused by the down-regulation of Ext1 expression. Our data provide the first evidence that Ext1 regulates the expression of HPSE and also indicated that levels of Ext1 and HPSE influenced the motility of FBJ cells.

AB - To know the involvement of glycosaminoglycans (GAGs) in the metastasis of mouse FBJ osteosarcoma cells, N α -lauroyl-O-(β-d- xylopyranosyl)-l-serinamide (Xyl-Ser-C12), which initiates elongation of GAG chains using the glycan biosynthesis system in cells, was administered to FBJ cells with different metastatic capacities. Production of glycosylated products derived from Xyl-Ser-C12, especially heparan sulfate (HS) GAG-type oligosaccharides such as GalNAc-GlcA-GlcNAc-GlcA-Gal-Gal-Xyl-Ser-C12, was indicated in poorly metastatic FBJ-S1 cells more than in highly metastatic FBJ-LL cells by LC-MS. The results of RT-PCR revealed that HS synthases, Ext1 and Ext2, were expressed in FBJ-S1 cells more than in FBJ-LL cells. Furthermore, siRNA against Ext1 suppressed the expression of HS and enhanced the motility of FBJ-S1 cells. In addition, the expression of heparanase (HPSE) was enhanced in Ext-1-knockdown FBJ-S1 cells, and responsible for the increase in cell motility caused by the down-regulation of Ext1 expression. Our data provide the first evidence that Ext1 regulates the expression of HPSE and also indicated that levels of Ext1 and HPSE influenced the motility of FBJ cells.

KW - Cell motility

KW - Ext1

KW - FBJ osteosarcoma

KW - Glycosaminoglycan

KW - Heparan sulfate

KW - Heparanase

KW - Saccharide primer

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U2 - 10.1007/s11010-012-1475-8

DO - 10.1007/s11010-012-1475-8

M3 - Article

C2 - 23054193

AN - SCOPUS:84871790084

SN - 0300-8177

VL - 373

SP - 63

EP - 72

JO - Enzymologia

JF - Enzymologia

IS - 1-2

ER -

Involvement of Ext1 and heparanase in migration of mouse FBJ osteosarcoma cells

Abstract

Keywords

ASJC Scopus subject areas

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